Abstract
A study was made to develop a new method for the production of flavine-adenine dinucleotide (FAD) from riboflavine and adenine by a mutant of Sarcina lutea deficient in the enzyme adenosine deaminase. It was found that this strain could convert exogenously supplemented riboflavine to extracellular FAD. The yields of FAD were increased by addition of D-cycloserine in the culture medium. The culture conditions for FAD production were investigated under the addition of D-cycloserine, and increased production of FAD was observed with the addition of an appropriate amount of thiamine, acetate, and sodium ion. The yield of 0.7 g/liter was obtained in the optimal culture in 5 days. Accumulated FAD was readily isolated by adsorption chromatography and ion-exchange chromatography in a 70% yield.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hirata M., Hayaishi O. Adenyl cyclase of Brevibacterium liquefaciens. Biochim Biophys Acta. 1967 Nov 21;149(1):1–11. doi: 10.1016/0005-2787(67)90685-5. [DOI] [PubMed] [Google Scholar]
- KULKA R. G. Colorimetric estimation of ketopentoses and ketohexoses. Biochem J. 1956 Aug;63(4):542–548. doi: 10.1042/bj0630542. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lambert M. P., Neuhaus F. C. Mechanism of D-cycloserine action: alanine racemase from Escherichia coli W. J Bacteriol. 1972 Jun;110(3):978–987. doi: 10.1128/jb.110.3.978-987.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TSUKIHARA K., MINOURA K., IZUMIYA M. Studies on the industrial production of flavin-adenine dinucleotide. I. Preparation of mycelium of Eremothecium ashbyii as a raw material containing flavin-adenine dinucleotide. J Vitaminol (Kyoto) 1960 Mar 10;6:68–76. doi: 10.5925/jnsv1954.6.68. [DOI] [PubMed] [Google Scholar]