Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1974 Mar;27(3):570–574. doi: 10.1128/am.27.3.570-574.1974

Short-Chain Acids of Pseudomonas Species Encountered in Clinical Specimens

C Wayne Moss 1, S B Samuels 1
PMCID: PMC380086  PMID: 4596388

Abstract

The short-chain acids of 36 strains of Pseudomonas grown on Trypticase soy agar were determined by gas-liquid chromatography. Distinct acid profiles were observed for each of the eight species tested. Propionic, isobutyric, and isovaleric acids were the principal acids detected in media extracts of P. maltophilia, P. cepacia, P. pseudoalcaligenes, P. diminuta, and P. vesiculare. The presence and relative amounts of the isobutyric and isovaleric acids clearly distinguished P. maltophilia, P. pseudoalcaligenes, and P. cepacia from other species. P. diminuta could be distinguished from P. vesiculare by the production of glutaric acid; P. testosteroni was the only species tested which produced relatively large amounts of phenylacetic acid.

Full text

PDF
570

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballard R. W., Doudoroff M., Stanier R. Y., Mandel M. Taxonomy of the aerobic psuedomonads: Pseudomonas diminuta and P. vesiculare. J Gen Microbiol. 1968 Oct;53(3):349–361. doi: 10.1099/00221287-53-3-349. [DOI] [PubMed] [Google Scholar]
  2. Brooks J. B., Weaver R. E., Tatum H. W., Billingsley S. A. Differentiation between Pseudomonas testosteroni and P. acidovorans by gas chromatography. Can J Microbiol. 1972 Sep;18(9):1477–1482. doi: 10.1139/m72-226. [DOI] [PubMed] [Google Scholar]
  3. Gilardi G. L. Characterization of Pseudomonas species isolated from clinical specimens. Appl Microbiol. 1971 Mar;21(3):414–419. doi: 10.1128/am.21.3.414-419.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilardi G. L. Characterization of nonfermentative nonfastidious gram negative bacteria encountered in medical bacteriology. J Appl Bacteriol. 1971 Sep;34(3):623–644. doi: 10.1111/j.1365-2672.1971.tb02326.x. [DOI] [PubMed] [Google Scholar]
  5. Gilardi G. L. Infrequently encountered Pseudomonas species causing infection in humans. Ann Intern Med. 1972 Aug;77(2):211–215. doi: 10.7326/0003-4819-77-2-211. [DOI] [PubMed] [Google Scholar]
  6. Klein M. M., Blazevic D. J. Nucleic acid enzyme studies of nonfermentative gram-negative bacteria using thin-layer chromatography. Appl Microbiol. 1972 Feb;23(2):276–280. doi: 10.1128/am.23.2.276-280.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lambert M. A., Moss C. W. Gas-liquid chromatography of short-chain fatty acids on Dexsil 300 GC. J Chromatogr. 1972 Dec 20;74(2):335–338. doi: 10.1016/s0021-9673(01)86164-1. [DOI] [PubMed] [Google Scholar]
  8. Moss C. W., Samuels S. B., Liddle J., McKinney R. M. Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia. J Bacteriol. 1973 Jun;114(3):1018–1024. doi: 10.1128/jb.114.3.1018-1024.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moss C. W., Samuels S. B., Weaver R. E. Cellular fatty acid composition of selected Pseudomonas species. Appl Microbiol. 1972 Oct;24(4):596–598. doi: 10.1128/am.24.4.596-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palleroni N. J., Ballard R. W., Ralston E., Doudoroff M. Deoxyribonucleic acid homologies among some Pseudomonas species. J Bacteriol. 1972 Apr;110(1):1–11. doi: 10.1128/jb.110.1.1-11.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pickett M. J., Pedersen M. M. Characterization of saccharolytic nonfermentative bacteria associated with man. Can J Microbiol. 1970 May;16(5):351–362. doi: 10.1139/m70-062. [DOI] [PubMed] [Google Scholar]
  12. Pickett M. J., Pedersen M. M. Salient features of nonsaccharolytic and weakly saccharolytic nonfermentative rods. Can J Microbiol. 1970 Jun;16(6):401–409. doi: 10.1139/m70-069. [DOI] [PubMed] [Google Scholar]
  13. Samuels S. B., Moss C. W., Weaver R. E. The fatty acids of Pseudomonas multivorans (Pseudomonas cepacia) and Pseudomonas kingii. J Gen Microbiol. 1973 Feb;74(2):275–279. doi: 10.1099/00221287-74-2-275. [DOI] [PubMed] [Google Scholar]
  14. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  15. Von Graevenitz A., Weinstein J. Pathogenic significance of Pseudomonas fluorescens and Pseudomonas putida. Yale J Biol Med. 1971 Dec;44(3):265–273. [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES