Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1974 Apr;27(4):671–673. doi: 10.1128/am.27.4.671-673.1974

Preparation of Free Heat-Resistant Ascospores from Byssochlamys Asci

H David Michener 1, A Douglas King Jr 1
PMCID: PMC380115  PMID: 4825977

Abstract

When Byssochlamys is grown for production of ascospores, some of the asci break up into their constituent ascospores, whereas others do not. For heat resistance studies, it is desirable to prepare a uniform suspension of free ascospores. This was accomplished with the aid of a pressure cell, from which a suspension of asci under high pressure was released to atmospheric pressure through a small orifice. Spores so treated had about the same heat resistance as untreated spores.

Full text

PDF
671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. King A. D., Michener H. D., Ito K. A. Control of Byssochlamys and Related Heat-resistant Fungi in Grape Products. Appl Microbiol. 1969 Aug;18(2):166–173. doi: 10.1128/am.18.2.166-173.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Partsch G. Simple method for the separation of ascospores. Appl Microbiol. 1969 Jun;17(6):925–925. doi: 10.1128/am.17.6.925-925.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Rousseau P., Halvorson H. O. Preparation and storage of single spores of Saccharomyces cerevisiae. J Bacteriol. 1969 Dec;100(3):1426–1427. doi: 10.1128/jb.100.3.1426-1427.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES