Abstract
Antisera to ten strains of mycobacteria in the Mycobacterium avium-Mycobacterium intracellulare group were obtained by injecting rabbits with ultraviolet light-killed cells. The antisera were conjugated with fluorescein isothiocyanate and used in the direct fluorescent antibody test. Individual antisera reacted specifically with the mycobacterial serotype used to produce them. The antisera were then combined in two multivalent pools. Each multivalent pool reacted specifically with its corresponding antigens. The multivalent antisera were thus found to provide a rapid identification method for the mycobacteria studied.
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- JONES W. D., Jr, SAITO H., KUBICA G. P. FLUORESCENT ANTIBODY TECHNIQUES WITH MYCOBACTERIA. Am Rev Respir Dis. 1965 Aug;92:255–260. doi: 10.1164/arrd.1965.92.2.255. [DOI] [PubMed] [Google Scholar]
- Jones W. D., Jr, Beam R. E., Kubica G. P. Fluorescent antibody techniques with Mycobacteria. II. Detection of Mycobacterium tuberculosis in sputum. Am Rev Respir Dis. 1967 Mar;95(3):516–517. doi: 10.1164/arrd.1967.95.3.516. [DOI] [PubMed] [Google Scholar]
- Martins R. R., Walker W. E., Batayias G. E., Gales P. W. The production of type specific fluorescent antibody for the identification of mycobacteria. Am Rev Respir Dis. 1973 Oct;108(4):979–982. doi: 10.1164/arrd.1973.108.4.979. [DOI] [PubMed] [Google Scholar]
- Schaefer W. B. Serologic identification of the atypical mycobacteria and its value in epidemiologic studies. Am Rev Respir Dis. 1967 Jul;96(1):115–118. doi: 10.1164/arrd.1967.96.1.115. [DOI] [PubMed] [Google Scholar]