Abstract
Antimicrobial activity of brilliant green dye in Trypticase soy broth (BBL) is reduced and ultimately destroyed by prolonged autoclaving at 121 C. Loss of antimicrobial activity is accompanied by decolorization of the dye. This is consistent with other evidence that antimicrobial activity of brilliant green resides in the colored dye ion. The dye is not decolorized when heated in distilled water or peptone, but is decolorized by heating in glucose, glycine, or sodium dodecyl sulfate, showing that decolorization results from reaction with components of the medium. To ensure optimal results, it is recommended that bacteriological media be sterilized by heat prior to addition of brilliant green dye.
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Selected References
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- Fung D. Y., Miller R. D. Effect of dyes on bacterial growth. Appl Microbiol. 1973 May;25(5):793–799. doi: 10.1128/am.25.5.793-799.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILLER V. R., BANWART G. J. EFFECT OF VARIOUS CONCENTRATIONS OF BRILLIANT GREEN AND BILE SALTS ON SALMONELLAE AND OTHER MICROORGANISMS. Appl Microbiol. 1965 Jan;13:77–80. doi: 10.1128/am.13.1.77-80.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moats W. A., Kinner J. A. Factors affecting selectivity of brilliant green-phenol red agar for salmonellae. Appl Microbiol. 1974 Jan;27(1):118–123. doi: 10.1128/am.27.1.118-123.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products. J Bacteriol. 1960 Dec;80:861–861. doi: 10.1128/jb.80.6.861-861.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paetzold O. H. Experimentelle Untersuchungen zur Frage des Wirkungsmechanismus von Brillantgrün auf Staphylokokken. II. Die Abhängigkeit der antibakteriellen Wirkung des Farbstoffes von seiner chemischen Konstitution. Arch Klin Exp Dermatol. 1966 Jan 11;224(1):104–116. [PubMed] [Google Scholar]
- STOKES J. L., OSBORNE W. W. A selenite brilliant green medium for the isolation of Salmonella. Appl Microbiol. 1955 Jul;3(4):217–220. doi: 10.1128/am.3.4.217-220.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]