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. 1974 May;27(5):878–885. doi: 10.1128/am.27.5.878-885.1974

Immobilized Aspartase-Containing Microbial Cells: Preparation and Enzymatic Properties

Ichiro Chibata 1, Tetsuya Tosa 1, Tadashi Sato 1
PMCID: PMC380170  PMID: 4208512

Abstract

The immobilization of asparatase-containing Escherichia coli was investigated by various methods, and the most active immobilized cells were obtained by entrapment in a polyacrylamide gel lattice. Other asparatase-containing bacteria were also entrapped by the same method, and the enzymatically active immobilized cells were obtained. The aspartase activity of the immobilized E. coli cells was increased nine- to tenfold by autolysis of the cells entrapped in the gel lattice. Enzymatic properties of the immobilized E. coli cells were investigated and compared with those of the intact cells. The optimal pH was 8.5 for the immobilized cells and 10.5 for the intact cells. The aspartase activities of immobilized and intact cells were not activated by Mn2+, which can activate the immobilized and native aspartases. The heat stability of the immobilized cells was somewhat higher than that of the intact cells. Bivalent metal ions such as Mn2+, Mg2+, Ca2+ protected against thermal inactivation of the aspartase activity of the immobilized and intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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