Abstract
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.
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Selected References
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- Chibata I., Tosa T., Sato T. Immobilized aspartase-containing microbial cells: preparation and enzymatic properties. Appl Microbiol. 1974 May;27(5):878–885. doi: 10.1128/am.27.5.878-885.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]