Abstract
Recent evaluations of the MacConkey agar test for differentiation of rapidly growing mycobacteria have revealed that certain strains of Mycobacterium fortuitum and M. chelonei that were expected to grow on MacConkey agar failed to do so. Investigation of two formulations of MacConkey agar showed that these two species grew better on the medium when the crystal violet dye was omitted. Several possible reasons for this difficulty are discussed. It is recommended that clinical laboratories engaged in differential identification of mycobacteria utilize commercial MacConkey agar without crystal violet when testing rapidly growing species of this genus.
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Selected References
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- CHAPMAN J. S., BERNARD J. S. The tolerances of unclassified mycobacteria. I. Limits of pH tolerance. Am Rev Respir Dis. 1962 Oct;86:582–583. doi: 10.1164/arrd.1962.86.4.582. [DOI] [PubMed] [Google Scholar]
- GORDON R. E., MIHM J. M. A comparison of four species of mycobacteria. J Gen Microbiol. 1959 Dec;21:736–748. doi: 10.1099/00221287-21-3-736. [DOI] [PubMed] [Google Scholar]
- JONES W. D., Jr, KUBICA G. P. THE DIFFERENTIAL TYPING OF CERTAIN RAPIDLY GROWING MYCOBACTERIA BASED ON THEIR SENSITIVITY TO VARIOUS DYES. Am Rev Respir Dis. 1963 Sep;88:355–359. doi: 10.1164/arrd.1963.88.3P1.355. [DOI] [PubMed] [Google Scholar]
- JONES W. D., Jr, KUBICA G. P. THE USE OF MACCONKEY'S AGAR FOR THE DIFFERENTIAL TYPING OF MYCOBACTERIUM FORTUITUM. Am J Med Technol. 1964 May-Jun;30:187–190. [PubMed] [Google Scholar]
- Kubica G. P., Baess I., Gordon R. E., Jenkins P. A., Kwapinski J. B., McDurmont C., Pattyn S. R., Saito H., Silcox V., Stanford J. L. A co-operative numerical analysis of rapidly growing mycobacteria. J Gen Microbiol. 1972 Nov;73(1):55–70. doi: 10.1099/00221287-73-1-55. [DOI] [PubMed] [Google Scholar]
- Kubica G. P. Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria. Am Rev Respir Dis. 1973 Jan;107(1):9–21. doi: 10.1164/arrd.1973.107.1.9. [DOI] [PubMed] [Google Scholar]
- Stanford J. L., Pattyn S. R., Portaels F., Gunthorpe W. J. Studies on Mycobacterium chelonei. J Med Microbiol. 1972 May;5(2):177–182. doi: 10.1099/00222615-5-2-177. [DOI] [PubMed] [Google Scholar]
- Wayne L. G., Doubek J. R. Diagnostic key to mycobacteria encountered in clinical laboratories. Appl Microbiol. 1968 Jun;16(6):925–931. doi: 10.1128/am.16.6.925-931.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]