Abstract
Microbiological conversion of drugs and natural products, including sterols and steroids, is an important component of the commercial preparation of these agents. We have developed methodology which allows for the production and selection of mutant organisms capable of specific desirable transformations. This methodology is based upon mutation of wild-type strains which are capable of completely degrading certain sterols and selection of the mutants blocked at the desired conversion. This procedure should be equally useful for many if not all drug classes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kozlova V. Kh, Fonina N. A. O kholesterinrazrushaiushchei aktivnosti nekotorykh vidov bakterii. Mikrobiologiia. 1971 Mar-Apr;40(2):275–279. [PubMed] [Google Scholar]
- Marsheck W. J., Kraychy S., Muir R. D. Microbial degradation of sterols. Appl Microbiol. 1972 Jan;23(1):72–77. doi: 10.1128/am.23.1.72-77.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SANTER M., AJL S. J., TURNER R. A. Steroid metabolism by a species of Pseudomonas. I. J Biol Chem. 1952 Sep;198(1):397–404. [PubMed] [Google Scholar]
- SIH C. J., LEE S. S., TSONG Y. Y., WANG K. C., CHANG F. N. AN EFFICIENT SYNTHESIS OF ESTRONE AND 19-NORSTEROIDS FROM CHOLESTEROL. J Am Chem Soc. 1965 Jun 20;87:2765–2766. doi: 10.1021/ja01090a048. [DOI] [PubMed] [Google Scholar]
- SIH C. J., WANG K. C. A NEW ROUTE TO ESTRONE FROM STEROLS. J Am Chem Soc. 1965 Mar 20;87:1387–1388. doi: 10.1021/ja01084a044. [DOI] [PubMed] [Google Scholar]
- Turfitt G. E. Microbiological Agencies in the Degradation of Steroids: II. Steroid Utilization by the Microflora of Soils. J Bacteriol. 1947 Nov;54(5):557–562. [PMC free article] [PubMed] [Google Scholar]
- Turfitt G. E. The microbiological degradation of steroids: 4. Fission of the steroid molecule. Biochem J. 1948;42(3):376–383. [PMC free article] [PubMed] [Google Scholar]