Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1972 Jan;23(1):135–140. doi: 10.1128/am.23.1.135-140.1972

New Pseudomonad Utilizing Methanol for Growth

Y Chalfan 1, R I Mateles 1
PMCID: PMC380291  PMID: 4110421

Abstract

A bacterium capable of rapid growth on methanol as sole carbon source was isolated and classified as a new pseudomonad. Its doubling time was about 100 min at 32 to 37 C, and it grew well at methanol concentrations up to 2%. The organism was sensitive to phosphate, but reasonable cell densities could be obtained by using pH control. Cell yields of about 31%, based on methanol consumed, were obtained. The amino acid pattern of the protein indicated that the bacterium holds promise as a source of single-cell protein.

Full text

PDF
135

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN L. R., STRAWINSKI R. J., MCCLESKEY C. S. THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI. Can J Microbiol. 1964 Oct;10:791–799. doi: 10.1139/m64-100. [DOI] [PubMed] [Google Scholar]
  2. Brown M. R., Foster J. H., Clamp J. R. Composition of Pseudomonas aeruginosa slime. Biochem J. 1969 May;112(4):521–525. doi: 10.1042/bj1120521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DWORKIN M., FOSTER J. W. Studies on Pseudomonas methanica (Söhngen) nov. comb. J Bacteriol. 1956 Nov;72(5):646–659. doi: 10.1128/jb.72.5.646-659.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Foster J. W., Davis R. H. A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria. J Bacteriol. 1966 May;91(5):1924–1931. doi: 10.1128/jb.91.5.1924-1931.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HARRINGTON A. A., KALLIO R. E. Oxidation of methanol and formaldehyde by pseudomonas methanica. Can J Microbiol. 1960 Feb;6:1–7. doi: 10.1139/m60-001. [DOI] [PubMed] [Google Scholar]
  6. HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
  7. Harder W., Quayle J. R. The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds. Biochem J. 1971 Mar;121(5):753–762. doi: 10.1042/bj1210753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heptinstall J., Quayle J. R. Pathways leading to and from serine during growth of Pseudomonas AM1 on C1 compounds or succinate. Biochem J. 1970 Apr;117(3):563–572. doi: 10.1042/bj1170563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KANEDA T., ROXBURGH J. M. A methanol-utilizing Bacterium. I. Description and nutritional requirements. Can J Microbiol. 1959 Feb;5(1):87–98. doi: 10.1139/m59-011. [DOI] [PubMed] [Google Scholar]
  10. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  11. LEADBETTER E. R., FOSTER J. W. Studies on some methane-utilizing bacteria. Arch Mikrobiol. 1958;30(1):91–118. doi: 10.1007/BF00509229. [DOI] [PubMed] [Google Scholar]
  12. PEEL D., QUAYLE J. R. Microbial growth on C1 compounds. I. Isolation and characterization of Pseudomonas AM 1. Biochem J. 1961 Dec;81:465–469. doi: 10.1042/bj0810465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STOCKS P. K., MCCLESKEY C. S. IDENTITY OF THE PINK-PIGMENTED METHANOL-OXIDIZING BACTERIA AS VIBRIO EXTORQUENS. J Bacteriol. 1964 Oct;88:1065–1070. doi: 10.1128/jb.88.4.1065-1070.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES