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. 1972 Jan;23(1):145–154. doi: 10.1128/am.23.1.145-154.1972

Characterization of the Growth of Herpes Simplex Virus in Human Lymphoid Cells

A Mizrahi a,1, J R Mitchen a, H W Von Heyden a, J Minowada a, G E Moore a
PMCID: PMC380293  PMID: 4110423

Abstract

Herpes simplex virus was grown in a 6-liter suspended culture of an atypical permanent human lymphoid cell line, Roswell Park Memorial Institute no. 8226. The kinetics of virus replication were determined by counting viruses by electron microscopy, plaque formation, and tissue culture infectivity. Deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase activity was determined during the course of infection. Electron microscopy studies substantiated the kinetics of the virus infection in lymphoid cells.

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Selected References

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