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. 1972 Feb;23(2):236–249. doi: 10.1128/am.23.2.236-249.1972

Factors Influencing the Production of H and M Antigens by Histoplasma capsulatum: Development and Evaluation of a Shake Culture Procedure1

Hugh-Bert Ehrhard 1,2,2, Leo Pine 1,2
PMCID: PMC380325  PMID: 4622821

Abstract

Studies were undertaken to improve the production of histoplasmin for use in complement-fixation tests and in the determination of H and M antibodies. A shake culture method performed at 25 C was developed with a yeast-phase inoculum. Eight strains of Histoplasma were tested in three synthetic media to evaluate the effects of strain and medium on H and M antigen production. Intrastrain variation was negligible, and antigen production was reproducible. All of the strains produced H antigen; six strains produced both H and M antigens, and two produced only H antigen. The time of H and M antigen appearance varied with the medium; M antigen appearance was dependent upon the strain and medium used. Titers of M antigen appeared to be greater in stagnant culture.

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Selected References

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