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. 1972 Mar;23(3):559–564. doi: 10.1128/am.23.3.559-564.1972

Failure to Detect Cell-Associated Enterotoxin B in Staphylococcus aureus by Immunofluorescence

Arne Forsgren 1, Urban Forsum 1, Hans O Hallander 1
PMCID: PMC380387  PMID: 4623281

Abstract

Enterotoxin B-producing and -nonproducing Staphylococcus aureus strains showed cell fluorescence when tested with fluoresceinisothiocyanate-labeled rabbit anti-enterotoxin B globulin, probably as a result of a protein A-immunoglobulin G (Ig G) interaction. No cell-bound enterotoxin B could be detected by immunofluorescence using F(ab1)2-fragments of anti-enterotoxin B globulin. However, soluble enterotoxin B could be estimated by immunofluorescence. Approximately 1,000-fold more enterotoxin B was detected by immunodiffusion as an extracellular product in the media than could be detected in the cell fraction. The results show that intact Ig G is not suitable for the detection of antigens other than protein A on the cell surface of S. aureus in conventional immunofluorescence. For such purposes, the use of F(ab1)2-fragments of Ig G is recommended.

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Selected References

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