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. 1972 Apr;23(4):721–724. doi: 10.1128/am.23.4.721-724.1972

Stable Reagent for the Detection of Antibody to the Specific Fraction I Antigen of Yersinia pestis1

James H Rust Jr 1,2, Sanford Berman 1,2, William H Habig 1,2, John D Marshall Jr 1,2, Dan C Cavanaugh 1,2
PMCID: PMC380425  PMID: 5062977

Abstract

A stable hemagglutinating antigen for detection of fraction I (FR-I) antibody of Yersinia pestis (Pasteurella pestis) is described. The antigen was prepared by sensitizing tanned, pyruvaldehyde-treated sheep erythrocytes (PAT SRBC) with FR-I antigen. Preliminary standardization by titration of each lot of FR-I was required to minimize the effect of molecular heterogeneity of specific FR-I antigen and to eliminate nonspecific reactions caused by the presence of a minor antigenic contaminant. In tests with sera from rabbits, dogs, and humans, FR-I PAT SRBC were as reactive as the previously employed standard antigen, FR-I-sensitized tanned erythrocytes. Fluid suspensions of FR-I PAT SRBC stored at 4 C for 3 months, or lyophilized preparations stored at ambient temperature for 6 months, showed no loss in antigenic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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