. Author manuscript; available in PMC: 2013 Oct 21.

Published in final edited form as: Virology. 2011 Dec 5;422(2):393–401. doi: 10.1016/j.virol.2011.11.004

Table 1.

Steady-state kinetics of WT and residue 114 mutant HIV-1 RT proteins with dTTP and acyTTP.

Enzyme	dNTPs	^Ŧ k_cat (min^-1)	K_m (μM) (Fold Change Compared to WT)	^Ŧ k_cat/K_m (μM^-1·min^-1)
WT	dTTP	1.91 ± 0.09	0.29 ± 0.07 (1×)	6.61
WT	acyTTP	1.91 ± 0.05	0.97 ± 0.28 (1×)	1.97
A114S	dTTP	7.92 ± 0.26	5.36 ± 1.94 (18×)	1.48
A114S	acyTTP	7.74 ± 0.93	9.6 ± 2.73 (10×)	0.81
A114C	dTTP	12.95 ± 1.19	7.14 ± 2.73 (25×)	1.81
A114C	acyTTP	11.95 ± 0.56	2.54 ± 0.76 (3×)	4.70
A114V	dTTP	1.38 ± 0.02	80.60 ± 44.97 (278×)	0.02
A114V	acyTTP	1.13 ± 0.15	9.37 ± 3.05	0.12

^Ŧ

kcat = (Vmax/enzyme concentration) ×10^-3