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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Nature. 2013 Sep 11;502(7471):389–392. doi: 10.1038/nature12584

Figure 3. DNA synthesis during BIR is conservative.

Figure 3

a, Experimental system to assay BIR using dynamic molecular combing including the position of hybridization probes P1, P2 and P3. b, (i), BrdU incorporation in the recipient is expected from conservative BIR (red). (ii), Formation of half-crossovers in pif1Δ leads to short patches of BrdU in recipient. c, Donor and recipient chromosomes separated using PFGE. d, The summary of molecular combing analysis. e, The donors and recipients of wt (PIF1) and pif1Δ. Each molecule was hybridized with P1, P2, P3 probes (green tracts) and treated with anti-BrdU antibodies (red tracts).