Abstract
Sterilization and optimal staining of Mycobacterium tuberculosis in sputum smears was accomplished by heating at 65 C for 30 min followed by exposure for 10 min to 10% formaldehyde fumes.
Full text
PDFPage 826
. 1972 Apr;23(4):826. doi: 10.1128/am.23.4.826-826.1972
A Method to Render Unstained Mycobacterial Smears Safe for Storage or Shipment
E B Blair
1, W W Bretherton
1, A H Tull
1
E B Blair
1Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240
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W W Bretherton
1Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240
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A H Tull
1Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240
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1Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240
PMCID: PMC380445 PMID: 4111815