Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1972 May;23(5):914–918. doi: 10.1128/am.23.5.914-918.1972

Immunogenicity of Rabies Virus Inactivated by β-Propiolactone, Acetylethyleneimine, and Ionizing Irradiation

T J Wiktor 1,2, H G Aaslestad 1,2, M M Kaplan 1,2,1
PMCID: PMC380470  PMID: 5031561

Abstract

Ionizing radiation, β-propiolactone, and acetylethyleneimine were compared for their ability as virus-inactivating agents for the preparation of rabies vaccine. Each agent reduced viral infectivity exponentially; ionizing radiation also destroyed viral hemagglutinin. The vaccine prepared by ionizing radiation was equal or superior to that prepared by β-propiolactone in its ability to protect mice from rabies infection. The acetylethyleneimine-treated vaccine was a less potent immunogen.

Full text

PDF
914

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crick J., Brown F. Viral subunits for rabies vaccination. Nature. 1969 Apr 5;222(5188):92–92. doi: 10.1038/222092a0. [DOI] [PubMed] [Google Scholar]
  2. Gruber J. Purification, concentration, and inactivation of Venezuelan equine encephalitis virus. Appl Microbiol. 1970 Sep;20(3):427–432. doi: 10.1128/am.20.3.427-432.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KISSLING R. E., REESE D. R. ANTI-RABIES VACCINE OF TISSUE CULTURE ORIGIN. J Immunol. 1963 Sep;91:362–368. [PubMed] [Google Scholar]
  4. Kuwert E., Wiktor T. J., Sokol F., Koprowski H. Hemagglutination by rabies virus. J Virol. 1968 Dec;2(12):1381–1392. doi: 10.1128/jvi.2.12.1381-1392.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. POLLEY J. R. Factors influencing inactivation of infectivity and hemagglutinin of influenza virus by gamma radiation. Can J Microbiol. 1961 Aug;7:535–541. doi: 10.1139/m61-063. [DOI] [PubMed] [Google Scholar]
  7. Reitman M., Tribble H. R. Inactivation of Venezuelan Equine Encephalomyelitis Virus by gamma-Radiation. Appl Microbiol. 1967 Nov;15(6):1456–1459. doi: 10.1128/am.15.6.1456-1459.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schlumberger H. D., Wiktor T. J., Koprowski H. Antigenic and immunogenic properties of components contained in rabies virus-infected tissue culture fluids. J Immunol. 1970 Aug;105(2):291–298. [PubMed] [Google Scholar]
  9. Sedwick W. D., Wiktor T. J. Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. J Virol. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. Purification of rabies virus grown in tissue culture. J Virol. 1968 Aug;2(8):836–849. doi: 10.1128/jvi.2.8.836-849.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TRAUB F. B., FRIEDEMANN U., BRASCH A., HUBER W. High intensity electrons as a tool for preparation of vaccines. I. Preparation of rabies vaccine. J Immunol. 1951 Nov;67(5):379–384. [PubMed] [Google Scholar]
  12. Wiktor T. J., Sokol F., Kuwert E., Koprowski H. Immunogenicity of concentrated and purified rabies vaccine of tissue culture origin. Proc Soc Exp Biol Med. 1969 Jul;131(3):799–805. doi: 10.3181/00379727-131-33981. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES