Abstract
A new dip-inoculum method for quantitative urine culture is described which utilizes a dual-chambered plastic „paddle” housing both a general purpose and differential medium. Comparative bacterial counts of 1,000 clinical specimens using the pour plate and this device were identical in 82.9% and within a factor of five in 95.6%. The „paddle” detected all but 19 of 258 specimens (92.6%) with 100,000 or greater colonies per ml. This simple, convenient method should allow more extensive use of quantitative urine culture in the diagnosis and follow-up of patients with urinary tract infections in office practice. It should not be considered as a substitute for the more definitive pour plate method or for standard methods for characterization of bacteriological species when more exact information is required.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cohen S. N., Kass E. H. A simple method for quantitative urine culture. N Engl J Med. 1967 Jul 27;277(4):176–180. doi: 10.1056/NEJM196707272770403. [DOI] [PubMed] [Google Scholar]
- Guttmann D., Naylor G. R. Dip-slide: an aid to quantitative urine culture in general practice. Br Med J. 1967 Aug 5;3(5561):343–345. doi: 10.1136/bmj.3.5561.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KASS E. H. Asymptomatic infections of the urinary tract. Trans Assoc Am Physicians. 1956;69:56–64. [PubMed] [Google Scholar]
- MONZON O. T., ORY E. M., DOBSON H. L., CARTER E., YOW E. M. A comparison of bacterial counts of the urine obtained by needle aspiration of the bladder, catheterization and midstream-voided methods. N Engl J Med. 1958 Oct 16;259(16):764–767. doi: 10.1056/NEJM195810162591603. [DOI] [PubMed] [Google Scholar]
- Mackey J. P., Sandys G. H. Laboratory diagnosis of infections of the urinary tract in general practice by means of a dip-inoculum transport medium. Br Med J. 1965 Nov 27;2(5473):1286–1288. doi: 10.1136/bmj.2.5473.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RILEY H. D., Jr Evaluation of a method for detecting and following urinary tract infection in females without catheterization. J Lab Clin Med. 1958 Dec;52(6):840–848. [PubMed] [Google Scholar]