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. 1972 Aug;24(2):213–218. doi: 10.1128/am.24.2.213-218.1972

Epidemiology of Pseudomonas aeruginosa in a Burns Hospital: Evaluation of Serological, Bacteriophage, and Pyocin Typing Methods1

Paul Edmonds a,2, Raymond R Suskind a, Bruce G Macmillan a, Ian A Holder a
PMCID: PMC380584  PMID: 4627229

Abstract

In a retrospective study 36 cultures of Pseudomonas aeruginosa, isolated from patients with fatal Pseudomonas burn wound sepsis and from burned patients with nonfatal P. aeruginosa infections, were used to evaluate the consistency and reliability of serological, phage, and pyocin typing as epidemiological tools. Frequency distributions of positive reactions were analyzed by a computer in a 3-way chi-square test, and a high degree of consistency was demonstrated for each method. From these data, 75% of the cultures were differentiated by serological, 90% by phage, and 100% by pyocin typing. There was no significant difference among organisms isolated from fatal cases of burn wound sepsis and organisms from patients with nonfatal infections (χ2 = 0.3418; P = 0.9870). The combined typing system was a sensitive and reliable epidemiological tool for intraspecific differentiation of P. aeruginosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergan T. Typing of Pseudomonas aeruginosa by pyocine production. Acta Pathol Microbiol Scand. 1968;72(3):401–411. doi: 10.1111/j.1699-0463.1968.tb00453.x. [DOI] [PubMed] [Google Scholar]
  2. DARRELL J. H., WAHBA A. H. PYOCINE-TYPING OF HOSPITAL STRAINS OF PSEUDOMONAS PYOCYANEA. J Clin Pathol. 1964 May;17:236–242. doi: 10.1136/jcp.17.3.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Farmer J. J., 3rd, Herman L. G. Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity of pyocin and bacteriophage. Appl Microbiol. 1969 Nov;18(5):760–765. doi: 10.1128/am.18.5.760-765.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farmer J. J., 3rd Mnemonic for reporting bacteriocin and bacteriophage types. Lancet. 1970 Jul 11;1(7663):96–96. doi: 10.1016/s0140-6736(70)92663-2. [DOI] [PubMed] [Google Scholar]
  5. Fisher M. W., Devlin H. B., Gnabasik F. J. New immunotype schema for Pseudomonas aeruginosa based on protective antigens. J Bacteriol. 1969 May;98(2):835–836. doi: 10.1128/jb.98.2.835-836.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GOULD J. C., McLEOD J. W. A study of the use of agglutinating sera and phage lysis in the classification of strains of Pseudomonas aeruginosa. J Pathol Bacteriol. 1960 Apr;79:295–311. [PubMed] [Google Scholar]
  7. GRABER C. D., LATTA R., VOGEL E. H., Jr, BRAME R. Bacteriophage grouping of Pseudomonas aeruginosa, with special emphasis on lysotypes occurring in infected burns. Am J Clin Pathol. 1962 Jan;37:54–62. doi: 10.1093/ajcp/37.1.54. [DOI] [PubMed] [Google Scholar]
  8. HABS I. Untersuchungen über die O-Antigene von Pseudomonas aeruginosa. Z Hyg Infektionskr. 1957;144(3):218–228. [PubMed] [Google Scholar]
  9. Hummel R. P., MacMillan B. G., Altemeier W. A. Topical and systemic antibacterial agents in the treatment of burns. Ann Surg. 1970 Sep;172(3):370–384. doi: 10.1097/00000658-197009000-00007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MacMillan B. G. Gentamicin in the management of thermal injuries. J Infect Dis. 1969 Apr-May;119(4):492–503. doi: 10.1093/infdis/119.4-5.492. [DOI] [PubMed] [Google Scholar]
  11. OSMAN M. A. PYOCINE TYPING OF PSEUDOMONAS AERUGINOSA. J Clin Pathol. 1965 Mar;18:200–202. doi: 10.1136/jcp.18.2.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. POSTIC B., FINLAND M. Observations on bacteriophage typing of Pseudomonas aeruginosa. J Clin Invest. 1961 Nov;40:2064–2075. doi: 10.1172/JCI104432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SUTTER V. L., HURST V., FENNELL J. A STANDARDIZED SYSTEM FOR PHAGE TYPING PSEUDOMONAS AERUGINOSA. Health Lab Sci. 1965 Jan;2:7–16. [PubMed] [Google Scholar]
  14. Sjöberg L., Lindberg A. A. Phage typing of Pseudomonas aeruginosa. Acta Pathol Microbiol Scand. 1968;74(1):61–68. doi: 10.1111/j.1699-0463.1968.tb03455.x. [DOI] [PubMed] [Google Scholar]
  15. TEPLITZ C., DAVIS D., MASON A. D., Jr, MONCRIEF J. A. PSEUDOMONAS BURN WOUND SEPSIS. I PATHOGENESIS OF EXPERIMENTAL PSEUDOMONAS BURN WOUND SEPSIS. J Surg Res. 1964 May;4:200–216. doi: 10.1016/s0022-4804(64)80026-3. [DOI] [PubMed] [Google Scholar]
  16. TEPLITZ C., DAVIS D., WALKER H. L., RAULSTON G. L., MASON A. D., Jr, MONCRIEF J. A. PSEUDOMONAS BURN WOUND SEPSIS. II HEMATOGENOUS INFECTION AT THE JUNCTION OF THE BURN WOUND AND THE UNBURNED HYPODERMIS. J Surg Res. 1964 May;4:217–222. doi: 10.1016/s0022-4804(64)80027-5. [DOI] [PubMed] [Google Scholar]
  17. VERDER E., EVANS J. A proposed antigenic schema for the identification of strains of Pseudomonas aeruginosa. J Infect Dis. 1961 Sep-Oct;109:183–193. doi: 10.1093/infdis/109.2.183. [DOI] [PubMed] [Google Scholar]
  18. WAHBA A. H. THE PRODUCTION AND INACTIVATION OF PYOCINES. J Hyg (Lond) 1963 Dec;61:431–441. doi: 10.1017/s0022172400021057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zabransky R. J., Day F. E. Pyocine typing of clinical strains of Pseudomonas aeruginosa. Appl Microbiol. 1969 Feb;17(2):293–296. doi: 10.1128/am.17.2.293-296.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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