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. 1972 Aug;24(2):270–274. doi: 10.1128/am.24.2.270-274.1972

Use of Polyelectrolytes and Electron Microscopy for Detection of Viruses from Stool

R K Chaudhary 1, J C N Westwood 1
PMCID: PMC380593  PMID: 4341968

Abstract

Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at a pH range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal at pH 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaudhary R. K., Westwood J. C. Plaque assay of poliovirus in plastic chambers. Can J Microbiol. 1969 Nov;15(11):1301–1303. doi: 10.1139/m69-235. [DOI] [PubMed] [Google Scholar]
  2. Doane F. W., Anderson N., Zbitnew A., Rhodes A. J. Application of electron microscopy to the diagnosis of virus infections. Can Med Assoc J. 1969 Jun 14;100(22):1043–1049. [PMC free article] [PubMed] [Google Scholar]
  3. Herrmann E. C., Jr Rates of isolation of viruses from a wide spectrum of clinical specimens. Am J Clin Pathol. 1972 Feb;57(2):188–194. doi: 10.1093/ajcp/57.2.188. [DOI] [PubMed] [Google Scholar]
  4. Kelen A. E., Labzoffsky N. A. Variations in the prevalence of enterovirus infections in Ontario, 1956-1965. Can Med Assoc J. 1967 Sep 23;97(13):797–801. [PMC free article] [PubMed] [Google Scholar]
  5. Parks W. P., Melnick J. L., Queiroga L. T., Khan H. A. Studies of infantile diarrhea in Karachi, Pakistan. I. Collection, virus isolation and typing of viruses. Am J Epidemiol. 1966 Sep;84(2):382–395. doi: 10.1093/oxfordjournals.aje.a120651. [DOI] [PubMed] [Google Scholar]
  6. RAMOS-ALVAREZ M., OLARTE J. DIARRHEAL DISEASES OF CHILDREN. THE OCCURRENCE OF ENTEROPATHOGENIC VIRUSES AND BACTERIA. Am J Dis Child. 1964 Mar;107:218–231. doi: 10.1001/archpedi.1964.02080060220002. [DOI] [PubMed] [Google Scholar]
  7. Sorber C. A., Sagik B. P., Malina J. F., Jr Monitoring of low-level virus in natural waters. Appl Microbiol. 1971 Sep;22(3):334–338. doi: 10.1128/am.22.3.334-338.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. WALLIS C., MELNICK J. L. Magnesium chloride enhancement of cell susceptibility to poliovirus. Virology. 1962 Feb;16:122–132. doi: 10.1016/0042-6822(62)90287-8. [DOI] [PubMed] [Google Scholar]
  9. Wallis C., Grinstein S., Melnick J. L., Fields J. E. Concentration of viruses from sewage and excreta on insoluble polyelectrolytes. Appl Microbiol. 1969 Dec;18(6):1007–1014. doi: 10.1128/am.18.6.1007-1014.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wallis C., Melnick J. L., Fields J. E. Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes. Appl Microbiol. 1971 Apr;21(4):703–709. doi: 10.1128/am.21.4.703-709.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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