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. 1972 Oct;24(4):645–649. doi: 10.1128/am.24.4.645-649.1972

Rapid, Direct Fluorescent-Antibody Method for the Detection of Salmonellae in Food and Feeds

N F Insalata 1, C W Mahnke 1, W G Dunlap 1
PMCID: PMC380629  PMID: 4564047

Abstract

An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ellis E. M., Harrington R., Jr A direct fluorescent antibody test for Salmonella. Arch Environ Health. 1969 Dec;19(6):876–881. doi: 10.1080/00039896.1969.10666946. [DOI] [PubMed] [Google Scholar]
  2. Fantasia L. D. Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products. Appl Microbiol. 1969 Nov;18(5):708–713. doi: 10.1128/am.18.5.708-713.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
  4. Goepfert J. M., Hicks R. Immunofluorescent staining of Salmonella species with flagellar sera. Appl Microbiol. 1969 Oct;18(4):612–617. doi: 10.1128/am.18.4.612-617.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goepfert J. M., Mann M. E., Hicks R. One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods. Appl Microbiol. 1970 Dec;20(6):977–983. doi: 10.1128/am.20.6.977-983.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harrington R., Jr, Ellis E. M. Immunofluorescence technique for detection of Salmonellae in tissues of swine. Am J Vet Res. 1972 Feb;33(2):445–447. [PubMed] [Google Scholar]
  8. Harrington R., Jr, Ellis E. M., Mallinson E. T., Ranck M., Solee R. E. An evaluation of a fluorescent antibody technique for the detection of Salmonellae in animal by-products, feeds, and tissues. J Am Vet Med Assoc. 1970 Dec 1;157(11):1898–1900. [PubMed] [Google Scholar]
  9. Laramore C. R., Moritz C. W. Fluorescent-antibody technique in detection of salmonellae in animal feed and feed ingredients. Appl Microbiol. 1969 Mar;17(3):352–354. doi: 10.1128/am.17.3.352-354.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thomason B. M., Wells J. G. Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae. Appl Microbiol. 1971 Nov;22(5):876–884. doi: 10.1128/am.22.5.876-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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