Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1972 Nov;24(5):735–741. doi: 10.1128/am.24.5.735-741.1972

Interferon Production by Human Cells In Vitro

Celsa A Spina 1, R Shihman Chang 1, L Mishra 1, H Dean Golden 1
PMCID: PMC380655  PMID: 4344957

Abstract

The relative capacity of several types of human cells and tissue to produce interferon was studied. Types of cells and tissue included were fibroblasts from embryos, foreskins, and biopsied skins; amnion cells; peripheral leukocytes; established lymphoid cell lines; established heteroploid cell lines; and chorioamniotic membrane. When Newcastle disease virus was used as the inducer, fibroblasts and amnion cells produced more interferon per 106 cells than leukocytes, lymphoid cells, and heteroploid cells. Only minor variations in interferon-producing capacity were observed among fibroblasts from 36 persons. Culture passage level, cell concentration, and inducer were factors that significantly affected interferon production.

Full text

PDF
735

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CANTELL K., PAUCKER K. Studies on viral interference in two lines of HeLa cells. Virology. 1963 Jan;19:81–87. doi: 10.1016/0042-6822(63)90027-8. [DOI] [PubMed] [Google Scholar]
  2. CHANG R. S. M. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc Soc Exp Biol Med. 1954 Nov;87(2):440–443. doi: 10.3181/00379727-87-21406. [DOI] [PubMed] [Google Scholar]
  3. CHANY C. An interferon-like inhibitor of viral multiplication from malignant cells (the viral autoinhibition phenomenon). Virology. 1961 Apr;13:485–492. doi: 10.1016/0042-6822(61)90279-3. [DOI] [PubMed] [Google Scholar]
  4. Cantell K., Strander H., Saxén L., Meyer B. Interferon response of human leukocytes during intrauterine and postnatal life. J Immunol. 1968 Jun;100(6):1304–1309. [PubMed] [Google Scholar]
  5. Carter W. A., Hande K. R., Essien B., Prochownik E., Kaback M. M. Comparative production of interferon by human fetal, neonatal, and maternal cells. Infect Immun. 1971 May;3(5):671–677. doi: 10.1128/iai.3.5.671-677.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chang R. S. Observations on the growth phases of human amnion cell cultures. J Natl Cancer Inst. 1968 Mar;40(3):491–503. [PubMed] [Google Scholar]
  7. Chang R. S. The loss of growth vitality of human lymphoid cell lines derived from healthy adults. Proc Soc Exp Biol Med. 1970 Nov;135(2):212–215. doi: 10.3181/00379727-135-35021. [DOI] [PubMed] [Google Scholar]
  8. Dianzani F., Baron S., Buckler C. E., Levy H. B. Mechanisms of DEAE-dextran enhancement of polynucleotide induction of interferon. Proc Soc Exp Biol Med. 1971 Apr;136(4):1111–1114. doi: 10.3181/00379727-136-35440. [DOI] [PubMed] [Google Scholar]
  9. Fournier F., Falcoff E., Chany C. Demonstration, mass production and characterization of a heavy molecular weight human interferon. J Immunol. 1967 Nov;99(5):1036–1041. [PubMed] [Google Scholar]
  10. GRESSER I. Production of interferon by suspensions of human leucocytes. Proc Soc Exp Biol Med. 1961 Dec;108:799–803. doi: 10.3181/00379727-108-27072. [DOI] [PubMed] [Google Scholar]
  11. Gergely L., Tóth F. D., Hadházy G., Szabó B. Enhanced interferon production in vitro by leucocytes from children with infectious mononucleosis. Acta Microbiol Acad Sci Hung. 1970;17(4):357–360. [PubMed] [Google Scholar]
  12. Gresser I., Lang D. J. Relationships between viruses and leucocytes. Prog Med Virol. 1966;8:62–130. [PubMed] [Google Scholar]
  13. HAYFLICK L. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. Exp Cell Res. 1965 Mar;37:614–636. doi: 10.1016/0014-4827(65)90211-9. [DOI] [PubMed] [Google Scholar]
  14. HO M., ENDERS J. F. Further studies on an inhibitor of viral activity appearing in infected cell cultures and its role in chronic viral infections. Virology. 1959 Nov;9:446–477. doi: 10.1016/0042-6822(59)90135-7. [DOI] [PubMed] [Google Scholar]
  15. Hadházy G., Gergely L., Nagy G., Tóth F. D. Comparison of interferon production in vitro by leukocytes from healthy and polycythaemic persons. Acta Microbiol Acad Sci Hung. 1968;15(2):141–144. [PubMed] [Google Scholar]
  16. Johnson T. C., McLaren L. C. Plaque Development and Induction of Interferon Synthesis by RMC Poliovirus. J Bacteriol. 1965 Sep;90(3):565–570. doi: 10.1128/jb.90.3.565-570.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LEE S. H., OZERE R. L. PRODUCTION OF INTERFERON BY HUMAN MONONUCLEAR LEUCOCYTES. Proc Soc Exp Biol Med. 1965 Jan;118:190–195. doi: 10.3181/00379727-118-29794. [DOI] [PubMed] [Google Scholar]
  18. Lee S. H. Interferon production by human leukocytes in vitro: some biological characteristics. Appl Microbiol. 1969 Nov;18(5):731–735. doi: 10.1128/am.18.5.731-735.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lee S. H., vanRooyen C. E., Ozere R. L. Additional studies of interferon production by human leukemic leukocytes in vitro. Cancer Res. 1969 Mar;29(3):645–652. [PubMed] [Google Scholar]
  20. Marchenko V. I., Babaiants A. A., Pokidysheva L. N. Chelovecheskii aminoticheskii interferon. Vopr Virusol. 1968 Jul-Aug;13(4):436–441. [PubMed] [Google Scholar]
  21. Merigan T. C., Gregory D. F., Petralli J. K. Physical properties of human interferon prepared in vitro and in vivo. Virology. 1966 Aug;29(4):515–522. doi: 10.1016/0042-6822(66)90276-5. [DOI] [PubMed] [Google Scholar]
  22. Myers M. W., Friedman R. M. Potentiation of human interferon production by superinduction. J Natl Cancer Inst. 1971 Oct;47(4):757–764. [PubMed] [Google Scholar]
  23. POHJANPELTO P. Two different thermostable variants of poliovirus. Virology. 1961 Nov;15:231–236. doi: 10.1016/0042-6822(61)90353-1. [DOI] [PubMed] [Google Scholar]
  24. Rabson A. S., O'Conor G. T., Baron S., Whang J. J., Legallais F. Y. Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child. Int J Cancer. 1966 Jan;1(1):89–106. doi: 10.1002/ijc.2910010112. [DOI] [PubMed] [Google Scholar]
  25. Ray C. G. The ontogeny of interferon production by human leukocytes. J Pediatr. 1970 Jan;76(1):94–98. doi: 10.1016/s0022-3476(70)80136-6. [DOI] [PubMed] [Google Scholar]
  26. Sanders C. V., Jr, Luby J. P., Sanford J. P., Hull A. R. Suppression of interferon response in lymphocytes from patients with uremia. J Lab Clin Med. 1971 May;77(5):768–776. [PubMed] [Google Scholar]
  27. Siewers C. M., John C. E., Medearis D. N., Jr Sensitivity of human cell strains to interferon. Proc Soc Exp Biol Med. 1970 Apr;133(4):1178–1183. doi: 10.3181/00379727-133-34650. [DOI] [PubMed] [Google Scholar]
  28. Solov'ev V. D., Bektermirov T. A., Vorotyntseva N. V., Fedorova Iu B., Kleimenova Z. N. Interferonoobrazovanie u detei zdorovykh i bol'nykh ostrymi respiratorynymi i énterovirusnymi infektsiiami. I. Produktsiia interferona vne organizma leikotsitami. Vopr Virusol. 1969 Jan-Feb;14(1):19–23. [PubMed] [Google Scholar]
  29. Solov'ev V. D., Pokidysheva L. N., Gutman N. R., Marchenko V. I., Kuznetsov V. P. Izuchenie proizvodstvennykh uslovii polucheniia chelovecheskogo leikotsitarnogo interferona. Vopr Virusol. 1969 Mar-Apr;14(2):148–154. [PubMed] [Google Scholar]
  30. St Geme J. W., Jr, Horrigan D. S. Comparative production of interferon by foreskin fibroblasts from newborn infants and children. J Lab Clin Med. 1969 Dec;74(6):946–949. [PubMed] [Google Scholar]
  31. Strander H., Cantell K., Leisti J., Nikkila E. Interferon response of lymphocytes in disorders with decreased resistance to infections. Clin Exp Immunol. 1970 Feb;6(2):263–272. [PMC free article] [PubMed] [Google Scholar]
  32. Strander H., Cantell K. Production of interferon by human leukocytes in vitro. Ann Med Exp Biol Fenn. 1966;44(2):265–273. [PubMed] [Google Scholar]
  33. Strander H. Production of interferon in serum-free human leukocyte suspensions. Appl Microbiol. 1969 Nov;18(5):810–815. doi: 10.1128/am.18.5.810-815.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tan Y. H., Armstrong J. A., Ke Y. H., Ho M. Regulation of cellular interferon production: enhancement by antimetabolites. Proc Natl Acad Sci U S A. 1970 Sep;67(1):464–471. doi: 10.1073/pnas.67.1.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Vilcek J., Rossman T. G., Varacalli F. Differential effects of actinomycin D and puromycin on the release of interferon induced by double stranded RNA. Nature. 1969 May 17;222(5194):682–683. doi: 10.1038/222682a0. [DOI] [PubMed] [Google Scholar]
  36. Wheelock E. F. Interferon-Like Virus-Inhibitor Induced in Human Leukocytes by Phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. doi: 10.1126/science.149.3681.310. [DOI] [PubMed] [Google Scholar]
  37. Worthington M., Aaronson S. A. Interferon system in cells from human tumors and from persons predisposed to cancer. Infect Immun. 1971 Mar;3(3):424–428. doi: 10.1128/iai.3.3.424-428.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Zajac B. A., Henle W., Henle G. Autogenous and virus-induced interferons from lines of lymphoblastoid cells. Cancer Res. 1969 Aug;29(8):1467–1475. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES