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. 1972 Dec;24(6):871–877. doi: 10.1128/am.24.6.871-877.1972

Effect of Low-Roughage Diets on the Microflora and Lipid Metabolism in the Rumen

M J Latham 1, J E Storry 1, M Elisabeth Sharpe 1
PMCID: PMC380689  PMID: 16349951

Abstract

Changing the diet of five lactating cows and one nonlactating cow from high to low roughage induced milk fat depression in the lactating cows and altered the composition of the rumen microflora. While the numbers of lactic and propionic acid-producing bacteria increased, the numbers of Butyrivibrio spp. decreased. The numbers of lipolytic bacteria and the in vitro lipolytic activity of the rumen fluid were also decreased, as was the extent of hydrogenation of linoleic and linolenic acids combined in soybean oil incubated in vitro with rumen fluid. It is suggested that among the bacterial population in the rumen the vibrios, which were adversely affected by the low-roughage diets, may contribute significantly to both lipolysis and hydrogenation in the rumen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chambers S. C. Pathogenic variation in Ophiobolus graminis. Aust J Biol Sci. 1970 Oct;23(5):1109–1113. [PubMed] [Google Scholar]
  3. Clarke D. G., Hawke J. C. Studies on rumen metabolism. VI. In vitro hydrolysis of triglyceride and isolation of a lipolytic fraction. J Sci Food Agric. 1970 Sep;21(9):446–452. doi: 10.1002/jsfa.2740210902. [DOI] [PubMed] [Google Scholar]
  4. Czerkawski J. W. Incubation inside the bovine rumen. Br J Nutr. 1967;21(4):865–878. doi: 10.1079/bjn19670087. [DOI] [PubMed] [Google Scholar]
  5. Davis C. L., Sachan D. S. Effect of feeding a milk fat depressing ration on fatty acid composition of blood lipids. J Dairy Sci. 1966 Dec;49(12):1567–1569. doi: 10.3168/jds.S0022-0302(66)88139-0. [DOI] [PubMed] [Google Scholar]
  6. Duncombe W. G. The colorimetric micro-determination of long-chain fatty acids. Biochem J. 1963 Jul;88(1):7–10. doi: 10.1042/bj0880007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Latham M. J., Sharpe M. E., Sutton J. D. The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation. J Appl Bacteriol. 1971 Jun;34(2):425–434. doi: 10.1111/j.1365-2672.1971.tb02302.x. [DOI] [PubMed] [Google Scholar]
  11. McCarthy R. D., Dimick P. S., Patton S. Field observations on the lipids of cows with depressed milk fat test. J Dairy Sci. 1966 Feb;49(2):205–209. doi: 10.3168/jds.S0022-0302(66)87830-X. [DOI] [PubMed] [Google Scholar]
  12. POLAN C. E., MCNEILL J. J., TOVE S. B. BIOHYDROGENATION OF UNSATURATED FATTY ACIDS BY RUMEN BACTERIA. J Bacteriol. 1964 Oct;88:1056–1064. doi: 10.1128/jb.88.4.1056-1064.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Slyter L. L., Oltjen R. R., Kern D. L., Blank F. C. Influence of type and level of grain and diethylstilbestrol on the rumen microbial populations of steers fed all-concentrate diets. J Anim Sci. 1970 Nov;31(5):996–1002. doi: 10.2527/jas1970.315996x. [DOI] [PubMed] [Google Scholar]
  14. Storry J. E., Rook J. A., Hall A. J. The effect of the amount and type of dietary fat on milk fat secretion in the cow. Br J Nutr. 1967;21(2):425–438. doi: 10.1079/bjn19670044. [DOI] [PubMed] [Google Scholar]
  15. Storry J. E., Rook J. A. The relationship in the cow between milk-fat secretion and ruminal volatile fatty acids. Br J Nutr. 1966;20(2):217–228. doi: 10.1079/bjn19660023. [DOI] [PubMed] [Google Scholar]
  16. Storry J. E., Sutton J. D. The effect of change from low-roughage to high-roughage diets on rumen fermentation, blood composition and milk fat secretion in the cow. Br J Nutr. 1969 Aug;23(3):511–521. doi: 10.1079/bjn19690060. [DOI] [PubMed] [Google Scholar]
  17. Storry J. E., Tuckley B. Thin-layer chromatography of plasma lipids by single development. Lipids. 1967 Nov;2(6):501–502. doi: 10.1007/BF02533180. [DOI] [PubMed] [Google Scholar]
  18. TOVE S. B., MATRONE G. Effect of purified diets on the fatty acid composition of sheep tallow. J Nutr. 1962 Mar;76:271–277. doi: 10.1093/jn/76.3.271. [DOI] [PubMed] [Google Scholar]
  19. Viviani R., Borgatti A. R., Matteuzzi D. Isolamento di tipici batteri ruminali attivi nella bioidrogenazione degli acidi grassi insaturi. Boll Soc Ital Biol Sper. 1968 Dec 31;44(24):2185–2189. [PubMed] [Google Scholar]
  20. Viviani R., Borgatti A. R., Monetti P. G., Mordenti A. Studies on long chain fatty acids composition in rumen fluid during the development of ruminal function in the calf. Zentralbl Veterinarmed A. 1967 Dec;14(9):833–844. doi: 10.1111/j.1439-0442.1967.tb00281.x. [DOI] [PubMed] [Google Scholar]
  21. Viviani R. Metabolism of long-chain fatty acids in the rumen. Adv Lipid Res. 1970;8:267–346. doi: 10.1016/b978-0-12-024908-4.50013-1. [DOI] [PubMed] [Google Scholar]
  22. Yokoyama M. T., Davis C. L. Hydrogenation of unsaturated fatty acids by Treponema (Borrelia) strain B 2 5, a rumen spirochete. J Bacteriol. 1971 Aug;107(2):519–527. doi: 10.1128/jb.107.2.519-527.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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