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. 1973 Jan;25(1):15–20. doi: 10.1128/am.25.1.15-20.1973

Characterization of Staphylococcus aureus in a Pediatric Burn Unit

Rodney F Smith 1, Carol L Bettge 1, Sandra L Dayton 1, James H Jorgensen 1
PMCID: PMC380727  PMID: 4568888

Abstract

A one-year study on an endemic strain of Staphylococcus aureus phage type 84/85 in a children's burn unit is described. The endemic strain rapidly colonized the burns and nares of acute patients after admission but was not isolated from a patient on admission. Nonendemic strains of S. aureus found on some new patients were mostly non-phage typable and did not prevail in burns. The endemic strain was rarely isolated from the nares and skin of reconstructive patients or from the nares of hospital personnel. The endemic strain did colonize the oral cavity, normal skin, and intestinal tract of some acute patients. Endemic and nonendemic strains of S. aureus from the burned children were compared in their biochemical activities and antibiotic sensitivities to two groups of S. aureus from one other local and one Danish burns unit. The latter groups of strains represented different combinations of staphylococcal phage group III strains. Each of the four groups of strains differed in production of hemolysins, Tween 80 hydrolysis, egg yolk reaction, and proteolysis of casein and gelatin. All of the strains were uniformly sensitive to gentamicin, oxacillin, and cephalothin. Only 4 of 162 strains tested were methicillin resistant. The endemic S. aureus strains of phage type 84/85 were uniformly resistant to eight other antibiotics including lincomycin and clindamycin. The endemic strain was not the known cause of a clinically documented infection in a group of 82 acute patients studied. The possible role of S. aureus strains of phage group III in burn grafting problems is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arbuthnott J. P., Gemmell C. G., Kent J., Lyell A. Haemolysin and enzyme patterns of coagulase-positive staphylococci isolated from toxic epidermal necrolysis, Ritter's disease and impetigo contagiosa. J Med Microbiol. 1969 Nov 4;2(4):479–487. doi: 10.1099/00222615-2-4-479. [DOI] [PubMed] [Google Scholar]
  2. Bülow P. Prevalence of extrachromosomal drug resistance. Staphylococci in Danish hospitals during the last decade: factors influencing some properties of predominant epidemic strains. Ann N Y Acad Sci. 1971 Jun 11;182:21–39. doi: 10.1111/j.1749-6632.1971.tb30640.x. [DOI] [PubMed] [Google Scholar]
  3. Chesbro W. R., Wamola I., Bartley C. H. Correlation of virulence with growth rate in Staphylococcus aureus. Can J Microbiol. 1969 Jul;15(7):723–729. doi: 10.1139/m69-127. [DOI] [PubMed] [Google Scholar]
  4. Klastersky J., Beumer J., Daneau D. Bacteriophage types and antibiotic susceptibility of Staphylococcus aureus. Appl Microbiol. 1971 Dec;22(6):1000–1007. doi: 10.1128/am.22.6.1000-1007.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lacey R. W., Alder V. G., Gillespie W. A. The surivival of Staphylococcus aureus on human skin. An investigation using mixed cultures. Br J Exp Pathol. 1970 Jun;51(3):305–313. [PMC free article] [PubMed] [Google Scholar]
  6. Marandon J. L., Oeding P. Investigations on animal Staphylococcus aureus strains. 1. Biochemical characteristics and phage typing. Acta Pathol Microbiol Scand. 1966;67(1):149–156. doi: 10.1111/apm.1966.67.1.149. [DOI] [PubMed] [Google Scholar]
  7. Marples R. R., Fulton J. E., Leyden J., McGinley K. J. Effect of antibiotics on the nasal flora in acne patients. Arch Dermatol. 1969 Jun;99(6):647–651. [PubMed] [Google Scholar]
  8. Martley F. G., Jayashankar S. R., Lawrence R. C. An improved agar medium for the detection of proteolytic organisms in total bacterial counts. J Appl Bacteriol. 1970 Jun;33(2):363–370. doi: 10.1111/j.1365-2672.1970.tb02208.x. [DOI] [PubMed] [Google Scholar]
  9. Smith R. F. Characterization of human cutaneous lipophilic diphtheroids. J Gen Microbiol. 1969 Mar;55(3):433–443. doi: 10.1099/00221287-55-3-433. [DOI] [PubMed] [Google Scholar]
  10. Smith R. F. Comparative enumeration of lipophilic and nonlipophilic cutaneous diphtheroids and cocci. Appl Microbiol. 1970 Feb;19(2):254–258. doi: 10.1128/am.19.2.254-258.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith R. F., Dayton S. L. Use of acetamide broth in the isolation of Pseudomonas aeruginosa from rectal swabs. Appl Microbiol. 1972 Jul;24(1):143–145. doi: 10.1128/am.24.1.143-145.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith R. F. Fatty acid requirements of human cutaneous lipophilic corynebacteria. J Gen Microbiol. 1970 Feb;60(2):259–263. doi: 10.1099/00221287-60-2-259. [DOI] [PubMed] [Google Scholar]
  13. Smith R. F., Jorgensen J. H., Bettge C. L., Dayton S. L. Evaluation of selective and differential media in the isolation and enumeration of airborne Staphylococcus aureus. Health Lab Sci. 1972 Oct;9(4):284–288. [PubMed] [Google Scholar]
  14. Thomsen M. The Burns Unit in Copenhagen. 10. Antibiotic sensitivity of Staphylococcus aureus isolated from burns. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):314–324. [PubMed] [Google Scholar]
  15. Thomsen M. The Burns Unit in Copenhagen. 11. Phage types of Staphylococcus aureus and the relation to antibiotic sensitivity. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):325–332. [PubMed] [Google Scholar]
  16. Thomsen M. The burns unit in Copenhagen. VII. Time of onset and duration of infection. Scand J Plast Reconstr Surg. 1970;4(1):61–66. doi: 10.3109/02844317009038445. [DOI] [PubMed] [Google Scholar]

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