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Three UIM regions in ataxin-3 interact with the parkin Ubl domain. Regions of the 1H–15N HSQC spectra of UIM_123 (73 μM) in the absence (black) and presence (cyan) of excess parkin Ubl domain. Resonances that experienced the largest chemical shift changes in UIM_123 upon addition of the parkin Ubl domain are labeled, and the direction of the chemical shift change is denoted with an arrow.
