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. 1973 Feb;25(2):266–269. doi: 10.1128/am.25.2.266-269.1973

Tissue Explants Used for Enhancement of Herpesvirus Isolations

S S Kalter 1,2, J Eichberg 1,2, R L Heberling 1,2, P J Felsburg 1,2
PMCID: PMC380784  PMID: 4351202

Abstract

Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENYESH-MELNICK M., ROSENBERG H. S., WATSON B. VIRUSES IN CELL CULTURES OF KIDNEYS OF CHILDREN WITH CONGENITAL HEART MALFORMATIONS AND OTHER DISEASES. Proc Soc Exp Biol Med. 1964 Nov;117:452–459. doi: 10.3181/00379727-117-29607. [DOI] [PubMed] [Google Scholar]
  2. Helmke R. J., Heberling R. L., Kalter S. S. Technique for viral neutralization antibody surveys in primary microcultures. Appl Microbiol. 1970 Dec;20(6):986–988. doi: 10.1128/am.20.6.986-988.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Horta-Barbosa L., Fuccillo D. A., London W. T., Jabbour J. T., Zeman W., Sever J. L. Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis. Proc Soc Exp Biol Med. 1969 Oct;132(1):272–277. doi: 10.3181/00379727-132-34196. [DOI] [PubMed] [Google Scholar]
  4. Howe C., Morgan C., Hsu K. C. Recent virologic applications of ferritin conjugates. Prog Med Virol. 1969;11:307–353. [PubMed] [Google Scholar]
  5. Kalter S. S., Armour V., Reinarz J. A. Rapid diagnosis of respiratory disease due to adenovirus and mycoplasma. Arch Gesamte Virusforsch. 1969;28(1):34–40. doi: 10.1007/BF01250843. [DOI] [PubMed] [Google Scholar]
  6. Kalter S. S., Felsburg P. J., Heberling R. L., Nahmias A. J., Brack M. Experimental Herpesvirus hominis type 2 infection in nonhuman primates. Proc Soc Exp Biol Med. 1972 Mar;139(3):964–968. doi: 10.3181/00379727-139-36276. [DOI] [PubMed] [Google Scholar]
  7. Kalter S. S., Heberling R. L. The study of simian viruses. WHO Chron. 1969 Mar;23(3):112–117. [PubMed] [Google Scholar]
  8. LIU C. Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):883–887. doi: 10.3181/00379727-92-22642. [DOI] [PubMed] [Google Scholar]
  9. Payne F. E., Baublis J. V., Itabashi H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. N Engl J Med. 1969 Sep 11;281(11):585–589. doi: 10.1056/NEJM196909112811103. [DOI] [PubMed] [Google Scholar]
  10. ROWE W. P., HUEBNER R. J., GILMORE L. K., PARROTT R. H., WARD T. G. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med. 1953 Dec;84(3):570–573. doi: 10.3181/00379727-84-20714. [DOI] [PubMed] [Google Scholar]
  11. Rogers N. G., Basnight M., Gibbs C. J., Gajdusek D. C. Latent viruses in chimpanzees with experimental kuru. Nature. 1967 Nov 4;216(5114):446–449. doi: 10.1038/216446a0. [DOI] [PubMed] [Google Scholar]
  12. WELLER T. H. Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster. Proc Soc Exp Biol Med. 1953 Jun;83(2):340–346. doi: 10.3181/00379727-83-20354. [DOI] [PubMed] [Google Scholar]

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