Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1973 Feb;25(2):279–283. doi: 10.1128/am.25.2.279-283.1973

Nonspecific Adsorption of Proteins to Microplates

Sakae Inouye a,1
PMCID: PMC380787  PMID: 4735108

Abstract

When a solution of purified adenovirus hexon proteins was diluted in polystyrene microplates without carrier proteins in the diluent, a higher rate of hexon adsorption to the microplates at higher dilutions took place. Addition of gelatin (0.01%), bovine serum albumin (0.01%), or calf serum (0.2%, vol/vol) to the diluent effectively prevented this adsorption. The adsorption to the microplates of adenovirions and measles virions was also prevented by the carrier proteins in the diluent. Certain additional features which distinguish the microtechnique of Takatsy from conventional macromethods are also discussed.

Full text

PDF
279

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askenase P. W., Leonard E. J. Solid phase radioimmunoassay of human beta 1C globulin. Immunochemistry. 1970 Jan;7(1):29–41. doi: 10.1016/0019-2791(70)90028-5. [DOI] [PubMed] [Google Scholar]
  2. Catt K., Tregear G. W. Solid-phase radioimmunoassay in antibody-coated tubes. Science. 1967 Dec 22;158(3808):1570–1572. doi: 10.1126/science.158.3808.1570. [DOI] [PubMed] [Google Scholar]
  3. Daugharty H., Warfield D. T., Davis M. L. Solid-phase radioimmunoassay of total and influenza-specific immunoglobulin G. Appl Microbiol. 1972 Feb;23(2):360–367. doi: 10.1128/am.23.2.360-367.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kochwa S., Brownell M., Rosenfield R. E., Wasserman L. R. Adsorption of proteins by polystyrene particles. I. Molecular unfolding and acquired immunogenicity of IgG. J Immunol. 1967 Nov;99(5):981–986. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Marusyk R. G., Norrby E. Association of deoxyribonuclease-sensitive material with adenovirus penton aggregates after treatment of infected cell cultures with sodium deoxycholate. Can J Microbiol. 1971 Aug;17(8):1009–1013. doi: 10.1139/m71-161. [DOI] [PubMed] [Google Scholar]
  7. Norrby E., Hammarskjöld B. Structural components of measles virus. Microbios. 1972 Jan;5(17):17–29. [PubMed] [Google Scholar]
  8. Norrby E., Skaaret P. The relationship between soluble antigens and the virion of adenovirus type 3. 3. Immunological identification of fiber antigen and isolated vertex capsomer antigen. Virology. 1967 Jul;32(3):489–502. doi: 10.1016/0042-6822(67)90301-7. [DOI] [PubMed] [Google Scholar]
  9. Norrby E., Wadell G., Marusyk H. Fiber-associated incomplete and complete hemagglutinins of adenovirus type 6. Arch Gesamte Virusforsch. 1969;28(2):239–244. doi: 10.1007/BF01249389. [DOI] [PubMed] [Google Scholar]
  10. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  11. Salmon S. E., Mackey G., Fudenberg H. H. "Sandwich" solid phase radioimmunoassay for the quantitative deterimination of human immunoglobulins. J Immunol. 1969 Jul;103(1):129–137. [PubMed] [Google Scholar]
  12. Sassen A., Kennes F. Influence of mixing on dilutions obtained with the microtiter technique. J Immunol Methods. 1972 May;1(3):313–315. doi: 10.1016/0022-1759(72)90009-9. [DOI] [PubMed] [Google Scholar]
  13. Shiroki K., Irisawa J., Shimojo H. Isolation and a preliminary characterization of temperature-sensitive mutants of adenovirus 12. Virology. 1972 Jul;49(1):1–11. doi: 10.1016/s0042-6822(72)80002-3. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES