Figure 2.
CID and LIT fragmentation of the Neu5Ac-Hex-GalNAc O-glycopeptide of HPX: A. MS1 spectrum of O-glycopeptide precursor ions of tryptic HPX digest; B. MS2 CID spectrum of major glycoform NeuAc-Hex-GalNAc (m/z 843.7, +4) with major de-glycosylated peptide ion (905.8) and peptide fragment ions (716.3 and 802.1); C. MS3 LIT spectrum of the de-glycosylated fragment (m/z 905.8, +3) with dominant and subdominant specific peptide fragmentsused for quantification.