Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1973 Mar;25(3):337–341. doi: 10.1128/am.25.3.337-341.1973

Turbidimetric Assay of Staphylococcal Nuclease

Alan Erickson 1, R H Deibel 1
PMCID: PMC380807  PMID: 4735446

Abstract

A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and heat-labile nuclease activity.

Full text

PDF
337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HOUCK J. C. The microdetermination of ribonuclease. Arch Biochem Biophys. 1958 Feb;73(2):384–390. doi: 10.1016/0003-9861(58)90283-2. [DOI] [PubMed] [Google Scholar]
  2. HOUCK J. C. The turbidimetric determination of deoxyribonuclease activity. Arch Biochem Biophys. 1959 May;82(1):135–144. doi: 10.1016/0003-9861(59)90098-0. [DOI] [PubMed] [Google Scholar]
  3. JEFFRIES C. D., HOLTMAN D. F., GUSE D. G. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957 Apr;73(4):590–591. doi: 10.1128/jb.73.4.590-591.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KUNITZ M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J Gen Physiol. 1950 Mar;33(4):349–362. doi: 10.1085/jgp.33.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. PRIVAT DE GARILHE M., CUNNINGHAM L., LAURILA U. R., LASKOWSKI M. Studies on isomeric dinucleotides derived from deoxyribonucleic acid. J Biol Chem. 1957 Feb;224(2):751–758. [PubMed] [Google Scholar]
  6. Smith P. B., Hancock G. A., Rhoden D. L. Improved medium for detecting deoxyribonuclease-producing bacteria. Appl Microbiol. 1969 Dec;18(6):991–993. doi: 10.1128/am.18.6.991-993.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Victor R., Lachica F., Weiss K. F., Deibel R. H. Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus. Appl Microbiol. 1969 Jul;18(1):126–127. doi: 10.1128/am.18.1.126-127.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. WECKMAN B. G., CATLIN B. W. Deoxyribonuclease activity of micrococci from clinical sources. J Bacteriol. 1957 Jun;73(6):747–753. doi: 10.1128/jb.73.6.747-753.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES