Table 1.

Primers for expression of recombinant Helicobacter hepaticus CDT fusion protein subunits.

Primers	Sequence (5′–3′)	Location in Cdt gene (bp)	PCR products size (bp)
cdtAF	CGGAATTCACACCAAAGGTTCATCAGCC†	54–696	642
cdtAR	CGGCTCGAGTTATTTAATGAAAAAAATAGG
cdtBF	CGGAATTCAATCTTGAAGATTATAGAG	770–1536	766
cdtBR	CCGCTCGAGCTAAAATCGTCCAAAATGC
cdtCF	CGGAATTCGGTGGTCCTGAAGATTTACCAG	1610–2098	488
cdtCF	CCGCTCGAGTTAAGGATTAATAGTTTTTGC

^†underlined sequences indicate restriction sites (5′-EcoRI and 3′-XhoI) for cloning into pET28(+) vector.