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Applied Microbiology logoLink to Applied Microbiology
. 1973 Apr;25(4):567–573. doi: 10.1128/am.25.4.567-573.1973

Comparison of Direct and Indirect Solid-Phase Microradioimmunoassays for the Detection of Viral Antigens and Antiviral Antibody

Joel D Rosenthal 1, Kozaburo Hayashi 1, Abner L Notkins 1
PMCID: PMC380863  PMID: 4349247

Abstract

Viral antigens were fixed to the surface of microtiter wells, and serial dilutions of antiviral antibody were added. The amount of antiviral antibody bound to viral antigens was determined by measuring the extent to which the antiviral antibody either inhibited the specific binding of 125I-labeled antiviral immunoglobulin G (IgG) (direct technique) or enhanced the specific binding of 125I-labeled anti-IgG (indirect technique). Immune complexes composed of viral antigens and antiviral antibody (human) could be detected by the binding of 125I-labeled rheumatoid factor. Specific binding was influenced by the concentration of protein in the diluents used during the different steps of the procedure. A high concentration of protein in the diluent used with the viral antigens decreased specific binding, whereas a high concentration of protein in the diluent used with 125I-labeled anti-IgG increased specific binding by decreasing nonspecific attachment of the labeled anti-IgG. Under the conditions employed, the titer of a given antiviral serum was several hundredfold greater by the indirect than by the direct technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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