Abstract
Evolution of 14CO2 by whole blood as well as by Diplococcus pneumoniae, Haemophilus sp., Pseudomonas aeruginosa, Pseudomonas diminuta, and Streptococcus pyogenes was examined by using the BACTEC system. The control medium was JLI no. 6A culture vial containing 30 ml of enriched tryptic soy broth and 1.5 μCi of 14C-substrate. Hypertonic media consisted of control medium with either 1 or 3% NaCl, 10% sucrose, and 5%, 10%, or 15% dextran. The most deleterious treatment to bacteria was 3% NaCl since it not only retarded 14CO2 production, but also prevented growth of D. pneumoniae, Haemophilus, and P. diminuta. The 10% sucrose treatment diminished 14CO2 output, although it did not retard growth of test organisms. This effect was probably due to 14C-substrate dilution rather than to osmotic effects. Dextran had slight effect on 14CO2 production and slightly acidified the medium. Of the treatments tested, only 10% sucrose reduced normal output of 14CO2 by whole blood. This also is probably due to 14C-substrate dilution. It appears that 10% sucrose is potentially the most useful osmotic agent for radiometric techniques since, although bacterial 14CO2 production is lowered, blood 14CO2 is lowered also.
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Selected References
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