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. 1973 Apr;25(4):636–640. doi: 10.1128/am.25.4.636-640.1973

Production of D-Alanine by Corynebacterium fascians

Shigeki Yamada 1, Haruko Maeshima 1, Mitsuru Wada 1, Ichiro Chibata 1
PMCID: PMC380876  PMID: 4699220

Abstract

A strain identified as Corynebacterium fascians was found to accumulate extracellular D-alanine from glycerol. Cultural conditions for the accumulation of D-alanine were investigated and, as a result, a yield of 7 g of D-alanine per liter was obtained after a 96-h incubation in a medium containing 5% glycerol, 4% (NH4)2HPO4, and 0.3% corn steep liquor. Optical purity of D-alanine was dependent upon the concentration of corn steep liquor. At the optimal condition, almost optically pure D-alanine was formed and readily isolated (5 g/liter) from the fermentation broth. The product was not contaminated with any detectable amount of other amino acids, except for glycine which was present at a concentration of less than 1 percent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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