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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Biochemistry. 2013 Aug 19;52(34):10.1021/bi400529q. doi: 10.1021/bi400529q

Figure 2.

Figure 2

The increase in stability (ΔΔG°obs, eq 1) upon titration of A-riboswitch (squares) or TLR RNA (circles) with chloride salts of Mg2+ (closed) or putrescine2+ (open). Tetraloop receptor data were collected in K•MOPS - EDTA buffer (see Materials and Methods) with 300 mM K+ while the A-riboswitch data were collected in the same buffer with 50 mM K+ and 20 μM DAP. Either MgCl2 or putrescine•2HCl was added to the concentration indicated by the abscissa, [2+].