Abstract
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 μg of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.
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Selected References
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- CASMAN E. P., BENNETT R. W. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD. Appl Microbiol. 1965 Mar;13:181–189. doi: 10.1128/am.13.2.181-189.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collins W. S., 2nd, Metzger J. F., Johnson A. D. A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin. J Immunol. 1972 Mar;108(3):852–856. [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gruber J., Wright G. G. Iodine-131 labeling of purified microbial antigens by microdiffusion. Proc Soc Exp Biol Med. 1967 Oct;126(1):282–284. doi: 10.3181/00379727-126-32425. [DOI] [PubMed] [Google Scholar]
- Johnson H. M., Bukovic J. A., Kauffman P. E., Peeler J. T. Staphylococcal enterotoxin B: solid-phase radioimmunoassay. Appl Microbiol. 1971 Nov;22(5):837–841. doi: 10.1128/am.22.5.837-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mann D., Granger H., Fahey J. L. Use of insoluble antibody for quantitative determination of small amounts of immunoglobulin. J Immunol. 1969 Mar;102(3):618–624. [PubMed] [Google Scholar]
- Robbins J. B., Haimovich J., Sela M. Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose. Immunochemistry. 1967 Jan;4(1):11–22. doi: 10.1016/0019-2791(67)90192-9. [DOI] [PubMed] [Google Scholar]
- Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
- Silverman S. J., Knott A. R., Howard M. Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods. Appl Microbiol. 1968 Jul;16(7):1019–1023. doi: 10.21236/ad0838753. [DOI] [PMC free article] [PubMed] [Google Scholar]