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. 1973 May;25(5):781–786. doi: 10.1128/am.25.5.781-786.1973

Selective Isolation of Leptospiras from Contaminated Material by Incorporation of Neomycin to Culture Media

Donald M Myers 1, Víctor M Varela-Díaz 1
PMCID: PMC380912  PMID: 4715558

Abstract

Incorporation of neomycin to the culture medium was found to be effective in inhibiting Escherichia coli contaminants without interfering with the growth of serotype L. autumnalis. The growth of 12 other Leptospira serotypes was unaffected by the addition of 300 μg of neomycin per ml to Ellinghausen medium or 5 μg/ml to Fletcher medium. Neomycin-containing medium was found to be of value in the isolation of leptospiras from cultures of blood from infected laboratory animals. A higher percentage of isolates was obtained in swine kidneys from an abattoir in medium containing neomycin than resulted from the same medium without antibiotic or with 5-fluorouracil. Contaminated leptospiral cultures growing in media with 5-fluorouracil were purified by subculturing into neomycin-containing media.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BABUDIERI B. Laboratory diagnosis of leptospirosis. Bull World Health Organ. 1961;24:45–58. [PMC free article] [PubMed] [Google Scholar]
  2. COUSINEAU J. G., McKIEL J. A. In vitro sensitivity of Leptospira to varioqs antimicrobial agents. Can J Microbiol. 1961 Oct;7:751–758. doi: 10.1139/m61-089. [DOI] [PubMed] [Google Scholar]
  3. ELLINGHAUSEN H. C., Jr, MCCULLOUGH W. G. NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: FRACTIONATION OF OLEIC ALBUMIN COMPLEX AND A MEDIUM OF BOVINE ALBUMIN AND POLYSORBATE 80. Am J Vet Res. 1965 Jan;26:45–51. [PubMed] [Google Scholar]
  4. JOHNSON R. C., ROGERS P. 5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE. J Bacteriol. 1964 Feb;87:422–426. doi: 10.1128/jb.87.2.422-426.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson R. C., Harris V. G. Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures. J Bacteriol. 1967 Jul;94(1):27–31. doi: 10.1128/jb.94.1.27-31.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stalheim O. H. Chemotherapy of renal leptospirosis in hamsters. Am J Vet Res. 1966 May;27(118):803–807. [PubMed] [Google Scholar]

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