Abstract
A nitrogen-deficient medium and m-Endo agar were employed in the isolation of members of the tribe Klebsielleae from surfaces of vegetables and seeds. With m-Endo agar at an incubation temperature of 37 C, nearly 50% of the vegetables and seven out of seven seed samples yielded organisms which biochemically and serologically were identified as Klebsiella pneumoniae, Viable counts were generally in the range of 103 cells per g of vegetable peel or seed. Organisms classified as K. pneumoniae exhibited seven different IMViC patterns, with the --++, ++++, and -+++ patterns most common. Seven of the eleven K. pneumoniae serotypes encountered have previously been isolated from human urinary tract and other infections. Fifty percent of the 40 K. pneumoniae examined exhibited positive acetylene-reducing activity, i.e., they possessed the capability for fixing N2. Vegetables containing K. pneumoniae may constitute a potential reservoir for human nosocomial genitourinary or other infections.
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Selected References
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- Bascomb S., Lapage S. P., Willcox W. R., Curtis M. A. Numerical classification of the tribe Klebsielleae. J Gen Microbiol. 1971 Jun;66(3):279–295. doi: 10.1099/00221287-66-3-279. [DOI] [PubMed] [Google Scholar]
- Bergersen F. J., Hipsley E. H. The presence of N2-fixing bacteria in the intestines of man and animals. J Gen Microbiol. 1970 Jan;60(1):61–65. doi: 10.1099/00221287-60-1-61. [DOI] [PubMed] [Google Scholar]
- Buchholz D. H., Young V. M., Friedman N. R., Reilly J. A., Mardiney M. R., Jr Bacterial proliferation in platelet products stored at room temperature. Transfusion-induced Enterobacter sepsis. N Engl J Med. 1971 Aug 19;285(8):429–433. doi: 10.1056/NEJM197108192850803. [DOI] [PubMed] [Google Scholar]
- COWAN S. T., STEEL K. J., SHAW C., DUGUID J. P. A classification of the Klebsiella group. J Gen Microbiol. 1960 Dec;23:601–612. doi: 10.1099/00221287-23-3-601. [DOI] [PubMed] [Google Scholar]
- Campbell N. E., Evans H. J. Use of Pankhurst tubes to assay acetylene reduction by facultative and anaerobic nitrogen-fixing bacteria. Can J Microbiol. 1969 Nov;15(11):1342–1343. doi: 10.1139/m69-243. [DOI] [PubMed] [Google Scholar]
- Edwards P. R. RELATIONSHIPS OF THE ENCAPSULATED BACILLI WITH SPECIAL REFERENCE TO BACT. AEROGENES. J Bacteriol. 1929 May;17(5):339–353. doi: 10.1128/jb.17.5.339-353.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eickhoff T. C., Steinhauer B. W., Finland M. The Klebsiella-Enterobacter-Serratia division. Biochemical and serologic characteristics and susceptibility to antibiotics. Ann Intern Med. 1966 Dec;65(6):1163–1179. doi: 10.7326/0003-4819-65-6-1163. [DOI] [PubMed] [Google Scholar]
- Eller C., Edwards F. F. Nitrogen-deficient medium in the differential isolation of Klebsiella and Enterobacter from feces. Appl Microbiol. 1968 Jun;16(6):896–899. doi: 10.1128/am.16.6.896-899.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GELDREICH E. E., KENNER B. A., KABLER P. W. OCCURRENCE OF COLIFORMS, FECAL COLIFORMS, AND STREPTOCOCCI ON VEGETATION AND INSECTS. Appl Microbiol. 1964 Jan;12:63–69. doi: 10.1128/am.12.1.63-69.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HINO S., WILSON P. W. Nitrogen fixation by a facultative bacillus. J Bacteriol. 1958 Apr;75(4):403–408. doi: 10.1128/jb.75.4.403-408.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hinshaw V., Punch J., Allison M. J., Dalton H. P. Frequency of R factor-mediated multiple drug resistance in Klebsiella and Aerobacter. Appl Microbiol. 1969 Feb;17(2):214–218. doi: 10.1128/am.17.2.214-218.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kayyali M. Z., Nicholson D. P., Smith I. M. A Klebsiela outbreak in a pediatric nursery: emergency action and preventive surveillance. Clin Pediatr (Phila) 1972 Jul;11(7):422–426. doi: 10.1177/000992287201100712. [DOI] [PubMed] [Google Scholar]
- MAHL M. C., WILSON P. W., FIFE M. A., EWING W. H. NITROGEN FIXATION BY MEMBERS OF THE TRIBE KLEBSIELLEAE. J Bacteriol. 1965 Jun;89:1482–1487. doi: 10.1128/jb.89.6.1482-1487.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsen J. M. Ten-minute test for differentiating between Klebsiella and Enterobacter isolates. Appl Microbiol. 1970 Mar;19(3):438–440. doi: 10.1128/am.19.3.438-440.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nunez W. J., Colmer A. R. Differentiation of Aerobacter-Klebsiella isolated from sugarcane. Appl Microbiol. 1968 Dec;16(12):1875–1878. doi: 10.1128/am.16.12.1875-1878.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osterman E., Rettger L. F. A Comparative Study of Organisms of the Friedländer and Coli-aerogenes Groups: II. Pathogenicity, Biochemical Reactions, and Serological Relationships. J Bacteriol. 1941 Dec;42(6):721–743. doi: 10.1128/jb.42.6.721-743.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramirez M. J. Differentiation of Klebsiella-Enterobacter (Aerobacter)-Serratia by biochemical tests and antibiotic susceptibility. Appl Microbiol. 1968 Oct;16(10):1548–1550. doi: 10.1128/am.16.10.1548-1550.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinhauer B. W., Eickhoff T. C., Kislak J. W., Finland M. The Klebsiella-Enterobacter-Serratia division. Clinical and epidemiologic characteristics. Ann Intern Med. 1966 Dec;65(6):1180–1194. doi: 10.7326/0003-4819-65-6-1180. [DOI] [PubMed] [Google Scholar]
- Zabransky R. J., Hall J. W., Day F. E., Needham G. M. Klebsiella, Enterobacter, and Serratia: biochemical differentiation and susceptibility to ampicillin and three cephalosporin derivatives. Appl Microbiol. 1969 Aug;18(2):198–203. doi: 10.1128/am.18.2.198-203.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]