Abstract
A method for improving the original Galton microtechnique for detecting leptospiral antibodies has been developed. Simultaneous titrations were performed on 281 animal and human sera and 17 hyperimmune sera with the microscopic agglutination (MA) test and the improved microtechnique. Reproducibility of the improved microtechnique was determined independently on 65 animal sera by two laboratory sections. The results obtained by comparing positive test data from human and animal sera indicated that agreement between the original MA test and this new method exceeded 94%, whereas the original Galton microtechnique and the original MA test agreed in a maximum of 77% of the tests. This study indicates that the results obtained with the improved microtechnique are much more comparable to results obtained with the original MA test than are those obtained with the original Galton microtechnique.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GALTON M. M., SULZER C. R., SANTAROSA C. A., FIELDS M. J. APPLICATION OF A MICROTECHNIQUE TO THE AGGLUTINATION TEST FOR LEPTOSPIRAL ANTIBODIES. Appl Microbiol. 1965 Jan;13:81–85. doi: 10.1128/am.13.1.81-85.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roessler W. G., Brewer C. R. Permanent turbidity standards. Appl Microbiol. 1967 Sep;15(5):1114–1121. doi: 10.1128/am.15.5.1114-1121.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sulzer C. R., Jones W. L. A modified semi-micro method for the test for leptospirosis. Health Lab Sci. 1973 Jan;10(1):13–17. [PubMed] [Google Scholar]