Table 2. Developmental accumulation of squalene in T1 greenhouse grown plants.
| Construct | Line | Leaf | Squalene |
|---|---|---|---|
| designation | development | (μg/g fw) | |
| Wild type (control) | # 21 | Young | 1.9 |
| Developing | 3.4 | ||
| Mature | 2.3 | ||
| Trichome | # 21 | Young | 1.3 |
| SQS | Developing | 4.8 | |
| cytosolic | Mature | 9.8 | |
| # 44 | Young | 3.6 | |
| Developing | 4.1 | ||
| Mature | 2.3 | ||
| Trichome | # 39 | Young | 7.1 |
| SQS | Developing | 16.7 | |
| plastidic | Mature | 26.3 | |
| Constitutive | # 16 | Young | 6.4 |
| SQS ? FPS | Developing | 5.5 | |
| cytosolic | Mature | 7.4 | |
| # 204 | Young | 2.6 | |
| Developing | 1.9 | ||
| Mature | 5.6 | ||
| Trichome | # 27 | Young | 5.7 |
| SQS + FPS | Developing | 8.4 | |
| cytosolic | Mature | 5.3 | |
| Constitutive | #7 | Young | 30.1 |
| SQS + FPS | Developing | 121.15 | |
| plastidic | Mature | 147.4 | |
| #15 | Young | 329.3 | |
| Developing | 450.4 | ||
| Mature | 667.5 | ||
| Trichome | #21 dwarf | Young | 90.0 |
| SQS + FPS | Developing | 74.3 | |
| plastidic | Mature | 256.7 | |
| #31 mosaic | Young | 527.6 | |
| Developing | 594.7 | ||
| Mature | 1,760.2 |
Individual, second-generation (T1) plants were propagated in the greenhouse and identified on the basis of preliminary chemical pro-filing screens. On average, young leaves were 5–7.5 cm in length, developing leaves were 10–15 cm in length, and mature leaves were greater than 20 cm long. Distinct phenotypes are noted for the plants harboring the constructs for trichome specifically expression of SQS and FPS targeted to the plastid compartment (see Fig. 3 for examples)