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M. citrifolia tolerance assay. Introgression flies from high and low tolerant lines were assayed for tolerance of ripe M. citrifolia fruit. Individual flies were exposed, and knockdown was observed between 30 and 60 min every 10 min. Flies used: D. simulans (susceptible background of introgression lines), D. sechellia (SynA tolerant line), high 8, low 10, 335-6, 197-6, and high 10 (recombinant lines and high and low tolerant lines).
