EFN-1 expression and tissue-specific rescue. (A) EFN-1::GFP (juIs52) expression in larvae and adults. EFN-1::GFP is expressed in a small number of anterior neurons, identified based on position and morphology as AIM, AIY, and AVK. (B) Tissue and cell-specific rescue of efn-1 axon guidance defects, scored in the efn-1(e96); kyIs104 background or in vab-1(e2027) as indicated. Asterisks indicate significant difference from efn-1 single mutant defects, by Fisher exact test. N > 50 except where indicated. *P < 0.05; **P < 0.01; ***P < 0.001. Bar, 10 μm.