Table 2. Candidate Eph signaling genes in C. elegans.
| Single mutant lateral axon phenotype (%) | Double vab-1(kd) mutant lateral axon phenotype (%) | Compound vs. vab-1(kd) (P-value) | Mammalian homolog | |
|---|---|---|---|---|
| vab-1(e118) | — | 12 | — | |
| Class I: suppressors of vab-1(kd) | ||||
| akt-1(ok525) | 0 | 3 | ***0.0009, N = 200 | Akt/PKB |
| sgk-1(ok538) | 0 | 3 | **0.002, N = 160 | SGK/serum-and glucocorticoid-inducible kinase |
| daf-16(mu86) | 0 | 3 | **0.003, N = 200 | FOXO |
| efn-3(ev696) | 0 | 3 | **0.008, N = 134 | Ephrin-A ligands |
| efn-2(ev658) | 0 | 4 | **0.006, N = 268 | Ephrin-A ligands |
| egl-19(ad695gf) | 0 | 4 | *0.024, N = 164 | VGCC α subunit |
| daf-18(ok480) | 0 | 4 | *0.036, N = 100 | PTEN |
| Class II: specific enhancers of vab-1(kd) | ||||
| efn-1(e96) | 26 | 33 | ***0.0001, N = 122 | Ephrin-A ligands |
| abl-1(ok171) 25° | 0 | 32 | **0.002, N = 100 | Abl/Abelson kinase |
| abl-1(ok171) | 1 | 22 | 0.091, N = 100 | Abl/Abelson kinase |
| aap-1(m889) 25° | 0 | 28 | *0.041, N = 50 | PI3K p50/p55 |
| age-1(hx546) 25° | 0 | 22 | 0.06, N = 130 | PI3K p110 |
| Class III: nonspecific enhancers of vab-1(kd) | ||||
| ngn-1(ok2200) | 32 | 50 | ***0.0001, N = 100 | Neurogenin |
| mig-10(ct41) | 2 | 22 | *0.05, N = 200 | Lamellipodin |
| Class IV: no significant change | ||||
| ina-1(gm144) | 3 | 11 | 0.825, N = 100 | alpha integrin subunit |
| git-1(tm1962) | 0 | 8 | 0.355, N = 100 | GIT1 |
| arf-6(tm1447) | 0 | 11 | 0.825, N = 100 | ARF6 |
| nck-1(ok694) | 0 | 14 | 0.834, N = 100 | NCK adapter protein |
| shc-1(ok198) | 0 | 8 | 0.347, N = 100 | Shc proteins |
| shc-2(tm328) | 1 | — | — | Shc proteins |
| ephx-1(ok494) | 0 | 15 | 0.683, N = 100 | Ephexin |
| rga-5(ok2241) | 0 | 12 | 1.0, N = 100 | Rho GTPase Activating protein |
| gap-2(tm748) | 0 | — | — | nGAP/synGAP |
| vpr-1(tm1411) | 0 | — | — | VAPB |
| wrk-1(ok695) | 0 | 9 | 0.490, N = 100 | Wrapper/Rega-1/Klingon |
| trk-1(tm3895)†† | 0 | 14 | 0.822, N = 72 | TRK neurotrophin receptor |
| trk-1(tm4054)†† | 0 | 6 | 0.139, N = 100 | TRK neurotrophin receptor |
| src-1(cj293) | 0 | — | — | Src family kinase member |
| src-2(ok819) | 0 | 12 | 1.0, N = 50 | Src family kinase member |
| egl-19(n2368cs) | 4 | 6 | 0.138, N = 100 | VGCC α subunit |
| jac-1(ok3000) | 0 | 5 | 0.125, N = 100 | p120 catenin |
| tag-341(ok1498) | 1 | 5 | 0.056, N = 200 | F-BAR and RhoGAP domains |
| akt-2(ok393) | 0 | 14 | 0.836, N = 100 | Akt/PKB |
| akt-2(tm812) | 0 | 18 | 0.326, N = 100 | Akt/PKB |
| lsy-6(ot71) | 0 | 19 | 0.248, N = 100 | N/A |
| cog-1(sy275)† | 5 | — | — | Nkx6 homeodomain protein |
| wsp-1(gm324) | 0 | 10 | 0.652, N = 100 | N-WASP |
| goa-1(sa734) | 1 | Sterile | — | Heterotrimeric G protein alpha subunit Go (Go/Gi class) |
| fmi-1(tm306) | 7 | — | — | Celsr/Flamingo |
| efn-4(bx80) | 3 | — | — | Ephrin-A ligands |
N, number of neurons scored. †cog-1 was tested with the Pgcy-7-GFP marker to label ASEL. ††trk-1(tm3985) is a 176-bp deletion, which removes most of exons 8 and 9 of the primary sequence and is predicted to truncate the protein between the transmembrane and kinase domain. tm4054 is an 823-bp deletion, which removes exons 6–9 and is predicted to be a null. Statistics, Fisher exact test: *, P < 0.05, **, P < 0.01, ***, P < 0.001.