Figure 2.
Knockdown of Ron suppresses tumor growth and delays metastasis. 1 × 106 Bx/vector and Bx/shRON cl.1 cells were implanted into the tail of pancreas of nude mice. Primary tumors and metastases were examined grossly and pathologically at 6–7 weeks after implantation. (a) Images of primary tumors (left) from Bx/vector and Bx/shRON cells and the distribution of tumor weights (right) from mice implanted with Bx/vector and Bx/shRON cells. The mean (n=15) of each group is represented by a horizontal line. (b) Representative photos show a normal liver from a mouse implanted with Bx/shRON cells and metastases to the liver from a mouse implanted with BxPC-3 vector control cells. The photos of H&E stained tissue sections show a tumor-free area of the liver, tumor infiltration into liver vessels (LVs) and metastases to a surrounding lymph node (LN) from Bx/vector tumors. The table summarized the total metastases of each group. (c) Representative photos of H&E stained Bx/vector and Bx/shRON tumors. (d) Immunohistochemistry staining of RON-β and MET of the orthotopic tumor section from Bx/vector and Bx/shRON cells. MET expression remains high in tumors generated from Bx/shRON cells.