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. Author manuscript; available in PMC: 2013 Nov 4.
Published in final edited form as: Science. 2013 May 24;340(6135):10.1126/science.1237874. doi: 10.1126/science.1237874

Fig. 4.

Fig. 4

Multiple MHC-II allomorphs can present type 2 epitopes to RhCMV/gag vector-elicited CD8+ T cells. (A) PBMC from a strain 68-1 RhCMV/gag-vaccinated RM (Rh22034) were incubated with SIVgag peptide-pulsed (and washed) RM3 cells (the MHC-II negative parental cell line) vs. RM3 transfectants expressing single Mamu-DR molecules, and then evaluated for peptide-specific CD8+ T cell recognition using flow cytometric ICS to detect induction of IFN-γ and/or TNF-α production (response frequencies are indicated in each quadrant). The Mamu-DR molecules tested included four that are expressed by Rh22034 (DRB1*0309, DRB1*0406, DRB5*0301, and DRB*w201), and one that is not expressed (DRB*w4:01). The SIVgag 15mer peptides tested corresponded to known MHC-II-blocked CD8+ T cell epitopes (Type 2) in this RM, except for Gag273-287 (15mer #69), which was MHC-I-blocked (Type 1), and therefore used as a negative control. (B) Similar analysis of the presentation of the MHC-II-blocked (Type 2) Gag221-235 (15mer #56) peptide to CD8+ T cells from two strain 68-1 RhCMV/gag vector-vaccinated RM (Rh22034 and Rh21836) by autologous B-lymphoblastoid cells, MHC-II null parental cells and single MHC-II transfectants corresponding to Mamu-DRB alleles that are reciprocally expressed by these 2 RM (expressed alleles denoted in red, non-expressed in black).