Skip to main content
NCBI home page
Genome Announcements logoLink to Genome Announcements
. 2013 Nov 7;1(6):e00914-13. doi: 10.1128/genomeA.00914-13

A Novel Ambisense Densovirus, Acheta domesticus Mini Ambidensovirus, from Crickets

Hanh T Pham 1, Qian Yu 1, Max Bergoin 1, Peter Tijssen 1,✉
PMCID: PMC3820778  PMID: 24201197

Abstract

The genome structure of Acheta domesticus mini ambidensovirus, isolated from crickets, resembled that of ambisense densoviruses from Lepidoptera but was 20% smaller. It had the highest (<25%) protein sequence identity with the nonstructural protein 1 (NS1) of Iteravirus and VP of Densovirus members (both with 25% coverage) and smaller (0.2- versus 0.55-kb) Y-shaped inverted terminal repeats.

GENOME ANNOUNCEMENT

The cricket industry has been devastated worldwide recently by the Acheta domesticus densovirus (AdDNV) (1–4). We also observed several, thus far unknown, viruses such as volvoviruses, which have circular, single-stranded DNA (ssDNA) genomes (5), and a new densovirus (parvovirus).

Two genera of insect parvoviruses, named densoviruses (6), are particularly relevant for this new densovirus. The Densovirus genus contains ambisense densoviruses from Lepidoptera, with genomes of 6 kb, Y-shaped inverted terminal repeats (ITRs) of about 0.55 kb, and sequence identities of about 85% (7–11). The Iteravirus genus contains monosense densoviruses, also from Lepidoptera, with 5-kb genomes, J-shaped 0.25-kb inverted terminal repeats (ITRs), and about 75% sequence identities (12–15).

A new virus with morphology and size similar to densoviruses was detected in some cricket samples from the United States. Virus was purified and DNA extracted as described previously (5). Digestion of viral DNA with EcoRI yielded 2 bands of about 700 bp and 4,200 bp on agarose gels. DNA was blunt ended with T4 DNA polymerase and a large Klenow fragment in the presence of dNTPs at room temperature (RT), ligated into the EcoRV site of pBlueskript KS(+), and transformed into SURE cells. DNA of clones with expected sizes were subcloned. Digestion with EcoNI within the terminal hairpins yielded clear reads of ITR sequences. Several complete clones were sequenced in both directions by use of Sanger’s primer-walking method as described previously (11). Contigs were assembled by use of the CAP3 program (http://pbil.univ-lyon1.fr/cap3.php/) (16).

Surprisingly, the genome structure and gene organization of this virus strongly resembled those of ambisense densoviruses from the Densovirus genus (7–11), but the genome sequence was only 4,945 nucleotides (nt) long, instead of about 6,035 nt, and lacked nucleotide sequence identity (best E value of 0.017, with a query coverage of 1%). Protein sequence identities were for the major nonstructural protein 1 (NS1) closest to Iteravirus members and, oddly, for the structural proteins (VP) closest to Densovirus members (both at best 25% identity for 25% coverage [or higher for shorter coverage]).

ITRs of AdMADV were smaller than those of densovirus members (199 versus about 545 nt) and Y-shaped, with a 113-nt hairpin. The 45-nt-long stem contained two side arms in the middle, nt 46 to 68, that occurred in two sequence orientations (flip/flop). It had a high GC content (63%) and contained inboard TATA boxes, at 193 to 199 for the NS cassette and at 4747 to 4753 for the VP cassette. This structure is identical to that of Densovirus ITRs.

The NS cassette consisted, as for Densovirus members, of NS3, followed by NS1 and an overlapping NS2. Splicing, as for Densovirus, would remove the NS3 open reading frame (ORF) and allow expression of NS1 and NS2 by leaky scanning. As for Densovirus, the putative splice acceptor site was located just upstream of the initiation codon of NS1 (1172-CAG/aATGNS1..N19..ATGNS2) (in GmDNV, 1395-CAG/ATGNS1..N4..ATGNS2). As for members of the Densovirus genus, the VP on the complementary strand also contained the phospholipase A2 motif (4,590 to 4,680 nt) (17) and the stop codons of NS1 and VP were neighbors (2661-TAG/AAT-2666), suggesting a small overlap of their transcripts, as for GmDNV.

Nucleotide sequence accession number.

The GenBank accession number of AdMADV is KF275669.

ACKNOWLEDGMENTS

This work was supported by the Natural Sciences and Engineering Research Council of Canada to P.T.; H.T.P. and Q.Y. acknowledge tuition fee waivers at INRS and scholarships from INRS and the People’s Republic of China.

Footnotes

Citation Pham HT, Yu Q, Bergoin M, Tijssen P. 2013. A novel ambisense densovirus, Acheta domesticus mini ambidensovirus, from crickets. Genome Announc. 1(6):e00914-13. doi:10.1128/genomeA.00914-13.

REFERENCES

  • 1. Liu K, Li Y, Jousset FX, Zadori Z, Szelei J, Yu Q, Pham HT, Lépine F, Bergoin M, Tijssen P. 2011. The Acheta domesticus densovirus, isolated from the European house cricket, has evolved an expression strategy unique among parvoviruses. J. Virol. 85:10069–10078 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2. Szelei J, Woodring J, Goettel MS, Duke G, Jousset FX, Liu KY, Zadori Z, Li Y, Styer E, Boucias DG, Kleespies RG, Bergoin M, Tijssen P. 2011. Susceptibility of North-American and European crickets to Acheta domesticus densovirus (AdDNV) and associated epizootics. J. Invertebr. Pathol. 106:394–399 [DOI] [PubMed] [Google Scholar]
  • 3. Weissman DB, Gray DA, Pham HT, Tijssen P. 2012. Billions and billions sold: pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make a potential disaster. Zootaxa 3504:67–88 [Google Scholar]
  • 4. Pham HT, Iwao H, Szelei J, Li Y, Liu K, Bergoin M, Tijssen P. 2013. Comparative genomic analysis of Acheta domesticus densoviruses from different outbreaks in Europe, North America, and Japan. Genome Announc. 1(4):e00629-13. 10.1128/genomeA.00629-13 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5. Pham HT, Bergoin M, Tijssen P. 2013. Acheta domesticus Volvovirus, a novel single-stranded circular DNA virus of the house cricket. Genome Announc. 1(2):e00079-13. 10.1128/genomeA.00079-13 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6. Tijssen P, Agbandje-McKenna M, Almendral JM, Bergoin M, Flegel TW, Hedman K, Kleinschmidt JA, Li Y, Pintel DJ, Tattersall P. 2011. Parvoviridae, p 375–395 In King AMQ, Adams MJ, Carstens E, Lefkowitz EJ. (ed), Virus taxonomy: classification and nomenclature of viruses: ninth report of the International Committee on Taxonomy of Viruses. Elsevier, San Diego, CA. [Google Scholar]
  • 7. Pham HT, Nguyen OT, Jousset FX, Bergoin M, Tijssen P. 2013. Junonia coenia densovirus (JcDNV) genome structure. Genome Announc. 1(4):e00591-13. 10.1128/genomeA.00591-13 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8. El-Far M, Szelei J, Yu Q, Fediere G, Bergoin M, Tijssen P. 2012. Organization of the ambisense genome of the Helicoverpa armigera densovirus. J. Virol. 86:7024. 10.1128/JVI.00865-12 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9. Huynh OT, Pham HT, Yu Q, Tijssen P. 2012. Pseudoplusia includens densovirus genome organization and expression strategy. J. Virol. 86:13127–13128 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10. Fédière G, El-Far M, Li Y, Bergoin M, Tijssen P. 2004. Expression strategy of densonucleosis virus from Mythimna loreyi. Virology 320:181–189 [DOI] [PubMed] [Google Scholar]
  • 11. Tijssen P, Li Y, El-Far M, Szelei J, Letarte M, Zádori Z. 2003. Organization and expression strategy of the ambisense genome of densonucleosis virus of Galleria mellonella. J. Virol. 77:10357–10365 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12. Yu Q, Hajek AE, Bergoin M, Tijssen P. 2012. Papilio polyxenes densovirus has an iteravirus-like genome organization. J. Virol. 86:9534–9535 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13. Yu Q, Fédière G, Abd-Alla A, Bergoin M, Tijssen P. 2012. Iteravirus-like genome organization of a densovirus from Sibine fusca Stoll. J. Virol. 86:8897–8898 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14. Fédière G, Li Y, Zádori Z, Szelei J, Tijssen P. 2002. Genome organization of Casphalia extranea densovirus, a new iteravirus. Virology 292:299–308 [DOI] [PubMed] [Google Scholar]
  • 15. Li Y, Zádori Z, Bando H, Dubuc R, Fédière G, Szelei J, Tijssen P. 2001. Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid. J. Gen. Virol. 82:2821–2825 [DOI] [PubMed] [Google Scholar]
  • 16. Huang X, Madan A. 1999. CAP3: A DNA sequence assembly program. Genome Res. 9:868–877 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17. Zádori Z, Szelei J, Lacoste MC, Li Y, Gariépy S, Raymond P, Allaire M, Nabi IR, Tijssen P. 2001. A viral phospholipase A2 is required for parvovirus infectivity. Dev. Cell 1:291–302 [DOI] [PubMed] [Google Scholar]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES