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. 1979 Jan;76(1):71–75. doi: 10.1073/pnas.76.1.71

Deletion mutants of simian virus 40 defective in biosynthesis of late viral mRNA.

C J Lai, G Khoury
PMCID: PMC382878  PMID: 218207

Abstract

Deletion mutants of simian virus 40 defective in late gene functions have been examined for genetic elements that control the biosynthesis of the viral mRNAs. Mutant-specific RNA from infected cells was identified by CH3Hg/(OH)/agarose gel electrophoresis and mapped by the nuclease S1 technique. Altered RNA species, shortened by a size equivalent to the deletion region, can be detected in cells infected with these mutants lacking sequences within the body RNA segments. On the other hand, mutants that lack the 5'-end of the body sequences (a splice junction) fail to accumulate the respective shortened RNA species. In particular, mutant dl-2301 whose deletion includes both the leader and body splice junctions plus the intervening sequences, exhibits a polar effect on a distal gene. Whereas the late region of dl-2301 can be transcribed normally, the mutant defect appears to be associated with little or no accumulation of the mutant-specific late RNA in the infected cells. These results suggest that splice junctions or the intervening sequences, or both, in the viral genome are control signals for post-transcriptional processing of the viral RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Dhar R., Laub O., Horowitz M., Khoury G. Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686–3690. doi: 10.1073/pnas.74.9.3686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  4. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  5. Brockman W. W., Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):942–946. doi: 10.1073/pnas.71.3.942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Celma M. L., Dhar R., Pan J., Weissman S. M. Comparison of the nucleotide sequence of the messenger RNA for the major structural protein of SV40 with the DNA sequence encoding the amino acids of the protein. Nucleic Acids Res. 1977 Aug;4(8):2549–2550. [PMC free article] [PubMed] [Google Scholar]
  7. Chou J. Y., Martin R. G. Complementation analysis of simian virus 40 mutants. J Virol. 1974 May;13(5):1101–1109. doi: 10.1128/jvi.13.5.1101-1109.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cole C. N., Landers T., Goff S. P., Manteuil-Brutlag S., Berg P. Physical and genetic characterization of deletion mutants of simian virus 40 constructed in vitro. J Virol. 1977 Oct;24(1):277–294. doi: 10.1128/jvi.24.1.277-294.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferdinand F. J., Brown M., Khoury G. Synthesis and characterization of late lytic simian virus 40 RNA from transcriptional complexes. Virology. 1977 May 1;78(1):150–161. doi: 10.1016/0042-6822(77)90087-3. [DOI] [PubMed] [Google Scholar]
  10. Gariglio P., Mousset S. Isolation and partial characterization of a nuclear RNA polymerase - SV40 DNA complex. FEBS Lett. 1975 Aug 1;56(1):149–155. doi: 10.1016/0014-5793(75)80130-x. [DOI] [PubMed] [Google Scholar]
  11. Gilbert W. Why genes in pieces? Nature. 1978 Feb 9;271(5645):501–501. doi: 10.1038/271501a0. [DOI] [PubMed] [Google Scholar]
  12. Hsu M. T., Ford J. Sequence arrangement of the 5' ends of simian virus 40 16S and 19S mRNAs. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4982–4985. doi: 10.1073/pnas.74.11.4982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lai C. J., Dhar R., Khoury G. Mapping the spliced and unspliced late lytic SV40 RNAs. Cell. 1978 Aug;14(4):971–982. doi: 10.1016/0092-8674(78)90351-3. [DOI] [PubMed] [Google Scholar]
  14. Lai C. J., Nathans D. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J Mol Biol. 1974 Oct 15;89(1):179–193. doi: 10.1016/0022-2836(74)90169-7. [DOI] [PubMed] [Google Scholar]
  15. Lai C. J., Nathans D. Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology. 1974 Aug;60(2):466–475. doi: 10.1016/0042-6822(74)90340-7. [DOI] [PubMed] [Google Scholar]
  16. Lai C. J., Nathans D. Mapping the genes of simian virus 40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):53–60. doi: 10.1101/sqb.1974.039.01.010. [DOI] [PubMed] [Google Scholar]
  17. Lai C. J., Nathans D. The B/C gene of simian virus 40. Virology. 1976 Dec;75(2):335–345. doi: 10.1016/0042-6822(76)90032-5. [DOI] [PubMed] [Google Scholar]
  18. Lavi S., Groner Y. 5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5323–5327. doi: 10.1073/pnas.74.12.5323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. May E., Kress M., May P. Characterization of two SV40 early mRNAs and evidence for a nuclear "prespliced" RNA species. Nucleic Acids Res. 1978 Sep;5(9):3083–3099. doi: 10.1093/nar/5.9.3083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reddy V. B., Thimmappaya B., Dhar R., Subramanian K. N., Zain B. S., Pan J., Ghosh P. K., Celma M. L., Weissman S. M. The genome of simian virus 40. Science. 1978 May 5;200(4341):494–502. doi: 10.1126/science.205947. [DOI] [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Tegtmeyer P., Ozer H. L. Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J Virol. 1971 Oct;8(4):516–524. doi: 10.1128/jvi.8.4.516-524.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weinberg R. A., Warnaar S. O., Winocour E. Isolation and characterization of simian virus 40 ribonucleic acid. J Virol. 1972 Aug;10(2):193–201. doi: 10.1128/jvi.10.2.193-201.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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