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. 1979 Jan;76(1):145–149. doi: 10.1073/pnas.76.1.145

Formation and properties of 1000-A-diameter, single-bilayer phospholipid vesicles.

H G Enoch, P Strittmatter
PMCID: PMC382893  PMID: 34148

Abstract

Two methods are reported for the formation of large, uniform-sized phospholipid vesicles. The methods involve the treatment of phospholipid, in the form of either small, sonicated vesicles or a dry lipid film, at a molar ratio of deoxycholate to phospholipid of 1:2. Subsequent removal of deoxycholate yields a stable preparation of vesicles. These vesicles are bounded by a single bilayer, have an average diameter of 1000 A, and are readily separated from sonicated vesicles (230 A) by gel filtration on Sepharose 4B. Since the 1000-A vesicles are capable of trapping enzymes and other macromolecules, they may prove valuable for the delivery of liposome-entrapped solutes to cells and for the localization of peptide segments of a spectrum of membrane-bound proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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