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. 1979 Feb;76(2):575–579. doi: 10.1073/pnas.76.2.575

Nucleotide sequence of Escherichia coli K-12 replication origin.

K Sugimoto, A Oka, H Sugisaki, M Takanami, A Nishimura, Y Yasuda, Y Hirota
PMCID: PMC382991  PMID: 370832

Abstract

From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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