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. 1979 Feb;76(2):640–644. doi: 10.1073/pnas.76.2.640

Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

M Rubinstein, S Rubinstein, P C Familletti, R S Miller, A A Waldman, S Pestka
PMCID: PMC383004  PMID: 284389

Abstract

A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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